Computational chemistry has recently introduced the improved reaction field theory and now is capable of evaluating the bulk solvent effect quite accurately. It is time to expand this capability to mixed solvents, as it is often extremely useful through is still not well understood. For example, the determination of rates of complexations of divalent metal ions with a tridentate metal-ion indic...

a new, simple, rapid, and cheap method for determination of trace amounts of nitrite ion is reported.determination is based on reduction of bromate ion by nitrite ion, in the presence of murexide inacidic media. during reaction of bromate ion with nitrite ion, the reaction mixture is decolorized.the absorbance changes is then monitored spectrophotometricaly at 520 nm, at 25 °c. dynamicrange of ...

Initial velocities of energy-dependent Ca++ uptake were measured in rat liver mitochondria by stopped flow techniques and dual wave length spectroscopy in the presence of the Ca++ indicator murexide. The initial rate of energy-dependent Ca++ uptake by mitochondria was 0.1 to 0.3 nmole per s per mg of protein at 5 to 15 pM Ca++, and increased to 8.2 nmoles of Ca++ per s per mg at 200 PM Ca++. Wh...

Ca(2+) uptake in mitochondrial fractions, isolated on Percoll discontinuous density gradients, from light- and dark-grown corn (Zea mays L. var W64A x W182E) shoots was characterized by dual wavelength spectroscopy and the Ca(2+)-sensitive dye murexide. In light-grown seedlings, the rate of mitochondrial Ca(2+) uptake was about 40 nanomoles per minute per milligram of mitochondrial protein. A p...

a mere turbiditj7 to a distinct precipitate. The degrees of albumosuria and albuminuria were not proportional to each other in all my cases. In a few cases there was a mere trace of albumose, while the quantity of albumen was large. What is called here albumose was characterized by the following tests :? (1) Not precipitated by heat. (2) Precipitated by HNoa. This precipitate is soluble on boil...

A new, simple, rapid, and cheap method for determination of trace amounts of nitrite ion is reported.Determination is based on reduction of bromate ion by nitrite ion, in the presence of murexide inacidic media. During reaction of bromate ion with nitrite ion, the reaction mixture is decolorized.The absorbance changes is then monitored spectrophotometricaly at 520 nm, at 25 °C. Dynamicrange of ...

Methods have been developed to isolate mitochondria from small amounts of normal and leukemic human lymphocytes obtained from small volumes of circulating blood. Mitochondrial respiratory functions were measured by polarographic techniques, and active calcium uptake was measured by a spectrophotometric procedure utilizing the calcium-sensitive dye murexide. The results indicate that the rates o...