نتایج جستجو برای: multiplex ligation dependentprobe amplification

تعداد نتایج: 97340  

Journal: :Haematologica 2006
Arjan Buijs Pieter Jaap Krijtenburg Ellen Meijer

We performed genomic profiling using multiplex ligation-dependent probe amplification (MLPA) in 54 cases with suspected or advanced chronic lymphocytic leukemia (CLL). MLPA detected abnormalities when the percentage of mutated cells was greater than approximately 35%. Loss of 9p21 CDNK2A/B was revealed. MLPA is an economically attractive, powerful tool in trial-based, centralized risk-assessmen...

2013
Chunxiu Gong Ming-Qiang Zhu Di Wu Bingyan Cao

Methods 20 SRS cases diagnosed in Beijing Children’s Hospital from 2006 to 2011 were studied retrospectively for clinical manifestations, physical signs, laboratory examinations and respond of GH treatment. We compared with 3 different diagnostic criteria and used the methylationspecific multiplex ligation dependent probe amplification (MS-MLPA) method to detect the chromosome 11p15 imprinting ...

Journal: :Neurology 2006
J C Mulley P Nelson S Guerrero L Dibbens X Iona J M McMahon L Harkin J Schouten S Yu S F Berkovic I E Scheffer

We examined cases of severe myoclonic epilepsy of infancy (SMEI) for exon deletions or duplications within the sodium channel SCN1A gene by multiplex ligation-dependent probe amplification. Two of 13 patients (15%) who fulfilled the strict clinical definition of SMEI but without SCN1A coding or splicing mutations had exonic deletions of SCN1A.

Journal: :international journal of reproductive biomedicine 0
mir davood omrani faezeh azizi masoumeh rajabibazl niloufar safavi naini sara omrani arezo mona abbasi

background: the major aneuploidies that are diagnosed prenatally involve the autosomal chromosomes 13, 18, and 21, as well as sex chromosomes, x and y. because multiplex ligation-dependent probe amplification (mlpa) is rapid and non-invasive, it has replaced traditional culture methods for the screening and diagnosis of common aneuploidies in some countries. objective:  to evaluate the sensitiv...

Journal: :Circulation 2011
Moniek G P J Cox Paul A van der Zwaag Christian van der Werf Jasper J van der Smagt Maartje Noorman Zahir A Bhuiyan Ans C P Wiesfeld Paul G A Volders Irene M van Langen Douwe E Atsma Dennis Dooijes Arthur van den Wijngaard Arjan C Houweling Jan D H Jongbloed Luc Jordaens Maarten J Cramer Pieter A Doevendans Jacques M T de Bakker Arthur A M Wilde J Peter van Tintelen Richard N W Hauer

BACKGROUND Arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C) is an autosomal dominant inherited disease with incomplete penetrance and variable expression. Causative mutations in genes encoding 5 desmosomal proteins are found in ≈50% of ARVD/C index patients. Previous genotype-phenotype relation studies involved mainly overt ARVD/C index patients, so follow-up data on relatives...

ژورنال: Hormozgan Medical Journal 2012
Khordadpoor-Deilamani, F, Noori Daloii, M.R,

Introduction: Lots of human diseases and syndromes result from partial or complete gene deletions and duplications or changes of certain specific chromosomal sequences. Many various methods are used to study the chromosomal aberrations including Comparative Genomic Hybridization (CGH), Fluorescent in Situ Hybridization (FISH), Southern blots, Multiplex Amplifiable Probe Hybridisation (MAP...

Journal: :Methods in molecular biology 2018
Cathy B Moelans Lilit Atanesyan Suvi P Savola Paul J van Diest

This chapter describes a method for the rapid assessment of promoter hypermethylation levels or methylation of imprinted regions in human genomic DNA extracted from various sources using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA). Multiplex ligation-dependent probe amplification (MLPA) is a powerful and easy-to-perform PCR-based technique that can identify g...

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