An attractive option for tissue engineering is to use of multicellular spheroids as microtissues, particularly with stem cell spheroids. Conventional approaches of fabricating spheroids suffer from low throughput and polydispersity in size, and fail to supplement cues from extracellular matrix (ECM) for enhanced differentiation. In this study, we report the application of microfluidics-generate...

Multicellular spheroids of cancer cells have been increasingly used to screen anti-tumor compounds, owing to their in vivo like microenvironment and structure as well as compatibility to high-throughput/high-content screening. Here we report the potency and efficacy of a family of bufadienolides to inhibit the growth of gastric cancer cell line HGC-27 in three-dimensional (3D) spheroidal models...

A macroscopic model of the tumor Gompertzian growth is proposed. This approach is based on the energetic balance among the different cell activities, described by methods of statistical mechanics and related to the growth inhibitor factors. The model is successfully applied to the multicellular tumor spheroid data.

Prediction and control of cancer invasion is a vital problem in medical science. This paper proposes a modern geometric Ricci–flow and entropy based model for control of avascular multicellular tumor spheroid growth and decay. As a tumor growth/decay control tool, a monoclonal antibody therapy is proposed.

Prediction and control of cancer invasion is a vital problem in medical science. This paper proposes a modern geometric Ricci–flow model for control of avascular multicellular tumor spheroid decay. As a decay control tool, a monoclonal antibody therapy is proposed.

This study presents a novel microfluidic approach for developing large scale screening assays of anticancer compounds on 3D multicellular spheroids. We have developed a microfluidic device with associated protocols that combine the high-throughput characteristics of droplet microfluidics for spheroid formation and aggregation with those of single-phase microfluidics for substance exchange, long...