Multiparameter flow cytometry is commonly used for the analysis of cells and droplet microreactors. However, their cost and complexity limits its applications in point-of-care systems. This paper demonstrates a high speed frequency multiplexed electronic detector for multicolor flow cytometry. The system can detect multiple fluorophores on a single PMT, rather than one per fluorophore. The dete...

We establish the behavior of the energy of minimizers of non-local Ginzburg-Landau energies with Coulomb repulsion in two space dimensions near the onset of multi-droplet patterns. Under suitable scaling of the background charge density with vanishing surface tension the non-local Ginzburg-Landau energy becomes asymptotically equivalent to a sharp interface energy with screened Coulomb interact...

Constructing a cell mimic is a major challenge posed by synthetic biologists. Efforts to this end have been primarily focused on lipid- and polymer-encapsulated containers, liposomes and polymersomes, respectively. Here, we introduce a multi-compartment, nested system comprising aqueous droplets stabilized in an oil/lipid mixture, all encapsulated in hydrogel. Functional capabilities (electrica...

The selective manipulation and incubation of individual picoliter drops in high-throughput droplet based microfluidic devices still remains challenging. We used a surface acoustic wave (SAW) to induce a bubble in a 3D designed multi-trap polydimethylsiloxane (PDMS) device to manipulate multiple droplets and demonstrate the selection, incubation and on-demand release of aqueous droplets from a c...

We propose a highly versatile and programmable nanolitre droplet-based platform that accepts an unlimited number of sample plugs from a multi-well plate, performs digitization of these sample plugs into smaller daughter droplets and subsequent synchronization-free, robust injection of multiple reagents into the sample daughter droplets on-demand. This platform combines excellent control of valv...

This study concerns high temperature flame synthesis of ceramic deposits utilizing a mono-disperse stream of droplets composed of an aqueous precursor. The droplets were injected into an oxy-acetylene flame. Mono-disperse droplets were generated with an electrostatic spray technique. High-speed droplet images suggest evidence of shedding of small particulates from the droplet surface as they en...

Experiments are reported on the combustion of free or unsupported methanol/dodecanol mixture droplets in a reduced gravity environment. The experiments were conducted in a drop tower. The Grashof number (based on drop diameter) in the moving frame of reference was on the order of 10 -5 for the period of droplet burning. Combustion of the mixture droplets was characterized by a multi-stage proce...

This paper reports a plug-based, microfluidic method for performing multi-step chemical reactions with millisecond time-control. It builds upon a previously reported method where aqueous reagents were injected into a flow of immiscible fluid (fluorocarbons) (H. Song et al., Angew. Chem. Int. Ed., 2003, 42, 768). The aqueous reagents formed plugs – droplets surrounded and transported by the immi...

This paper presents a novel hybrid particle-grid method that tightly couples Lagrangian particle approach with Eulerian grid approach to simulate multi-scale diffuse materials varying from disperse droplets to dissipating spray and their natural mixture and transition, originated from a violent (high-speed) liquid stream. Despite the fact that Lagrangian particles are widely employed for repres...