Objective: Evaluate the protective effect of artificial saliva formulations associated or not with mucin on dentin. Materials and Methods: Bovine dentin specimens were randomly allocated to 10 groups (n = 20) according tested presence absence mucin: Amaechi et al. (1998); Klimek (1982); Vieira (2005) Eisenburger (2001) deionized water (control). Samples submitted an erosive cycle consisting two...

Background: Pseudomyxoma peritonei (PMP) is a rare disease with excess intraperitoneal mucin secretion. Treatment involves laparotomy, cytoreduction and chemotherapy that is very invasive with patients often acquiring numerous compromises. Hence a mucolytic comprising of bromelain and N-acetyl cystein has been developed to solubilise mucin in situ for removal by catherization. Owing to differen...

Monoclonal antibodies against rabbit ocular mucin (ROM) and porcine stomach mucin (PSM) were developed to explore the biosynthesis and functional significance of ocular mucin. A nitrocellulose-based dot enzyme-linked immunosorbent assay (ELISA) was developed for hybridoma screening and mucin quantitation. The sensitivity was found to be at the level of 1 ng mucin per dot, which was about 1000 t...

Gram-negative bacteria can stimulate mucin production, but excessive mucus supports bacterial infection and consequently leads to airway obstruction. Therefore, the effect of dexamethasone (DEX) and the antioxidant acetyl-cysteine (ACC) on bacteria-induced mucus expression was investigated. Explanted human airway mucosa and mucoepidermoid cells (Calu-3) were stimulated with lipopolysaccharide (...

Monoclonal antibody TH1 (IgM) was prepared by immunizing mice with deglycosylated (TFMSA-treated) cystic fibrosis mucin. TH1 reacted strongly with TFMSA treated cystic fibrosis mucin but not with the fully glycosylated mucin, indicating reactivity with a core mucin epitope. TH1 showed no reactivity with ovine mucin (98% of glycans as sialyl-Tn) but reacted strongly with desialylated ovine mucin...

two sialic acid specific lectins episesarma tetragonum agglutinin–1 and 2 were purified from the hemolymph of the mangrove crab, episesarma tetragonum . the major lectin was purified using cnbr-activated sepharose 4b conjugated to fetuin. n-acetyl glucosamine containing buffer was used for elution. the hemagglutination activity of purified lectin was inhibited by glycoproteins containing siaα, ...

Loss of integrity of the epithelial/mucosal barrier in the small intestine has been associated with different pathologies that originate and/or develop in the gastrointestinal tract. We showed recently that mucin, the main protein in the mucus layer, is disrupted during early periods of intestinal ischemia. This event is accompanied by entry of pancreatic digestive enzymes into the intestinal w...

Abstract Mucinous carcinoma (MC) of the breast is a rare, specialized subtype invasive (IBC) accounting for approximately 1% to 4% all primary malignancies. occurs predominantly in patients who are postmenopausal or elderly. It usually detected on screening mammography, but occasionally patient may present with palpable mass. The most common mammographic appearance an equal high density, oval r...

Cell-surface mucin glycoproteins are altered with the onset of oncogenesis. Knowledge of mucin structure could be used in vaccine strategies that target tumor-associated mucin motifs. Thus far, however, mucins have resisted detailed molecular analysis. Reported herein is the solution conformation of a highly complex segment of the mucin CD43. The elongated secondary structure of the isolated mu...

Our model of the MUC2 mucin shows a well-organized netlike gel that is cross-linked by six different covalent and noncovalent bonds. When the MUC2 mucin is packed in the mucin granule it is organized by an amino-terminal concatenated ring platform formed at high calcium and low pH. This packing allows an ordered release and a normal mucin expansion when calcium is removed and pH increased by bi...