somatic embryogenesis was initiated in c. sativus l. from shoot meristems on ls medium containing bap (2×10-5 m)+naa (2×10-5 m). various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. matured embryos coul...

The gerbera market would benefit from an efficient and simple protocol for high rate regeneration for propagation and genetic engineering. With these objectives, this investigation was done on shoot regeneration via direct organogenesis from leafy petiole explants of Gerbera jamesonii ‘Royal Soft Pink’. Murashige and Skoog (1962) (MS) medium was supplemented with 0.1 mg L-1 indole-3-acetic acid...

three separate experiments were conducted to investigate micropropagation of four pear cultivars. in the first trial, the effect of four cultural media (ms, ms/2, msn/2 and mql) were compared on such traits as shoot length, shoot number and color of leaf. from among the media, ms/2 and msn/2 resulted in the most shoot proliferation and the longest shoots respectively. ms medium produced green d...

Leaves from in vitro proliferating shoots of cvs. Chandler and Hartley of Persian walnut were cultured on MS and NN media supplemented with BA and NAA. Three light conditions including darkness, low light intensity and modified light intensity were used. Most of the calli were friable and non-embryonic in all three conditions. Rate of callogenesis depended on cultivar, culture medium, light con...

A modern biotechnological technique to obtain useful natural products from plants is to isolate them from their callus cultures. Lallemantia iberica is an annual herb of the Lamiaceae family known for its stimulant, diuretic, and expectorant effects in Iranian folk medicine. The present study investigated the induction of plant callus tissue and identification of its volatile compounds. For thi...

various plant organ segments including segments from roots (root tip, and root collar), stems, leaves, and also seeds of several hyoscyamus species (i.e. h. pusillus, h. arachnoideus, annual and biennial h. niger from two distinct geographical regions) were cultured on ms media with various growth regulator combinations, to assess the ability of flower production. statistical assessments showed...

hyssop (hyssopus officinalis l.), belongs to the family of lamiaceae which its origin is asia minor, and is considered as one of the most important medicinal plant. this experiment was done to study the effects of population and bap concentrations on hyssop plant regeneration under in vitro culture conditions. this research were performed in factorial experiment with three populations (hamadan,...

lisiantus is introduced among the 10 top cutting flowers in the world. micropropagation can be used in this plant because of some problems in traditional propagation. in this research, the effects of the growth regulators on the in vitro shoot regeneration and rooting of the shoots obtained from nodal explants were investigated. the effects of the different concentrations of bap and kin on shoo...

Various plant organ segments including segments from roots (root tip, and root collar), stems, leaves, and also seeds of several Hyoscyamus species (i.e. H. pusillus, H. arachnoideus, annual and biennial H. niger from two distinct geographical regions) were cultured on MS media with various growth regulator combinations, to assess the ability of flower production. Statistical assessments showed...