Abstract Fish transportation is a regular farming and experimental practice that mainly results in stress conditions may extend to induce high mortality rate. In the present simulation, Oreochromis niloticus were transported for 2 h maintained another 6 containers assess effect of tranquilization with tricaine methanesulfonate (MS-222) and/or addition iodine on rate restoring normal physiologic...

In wild-type trimethylamine dehydrogenase, residue Arg-222 is positioned close to the isoalloxazine N1/C2 positions of the 6S-cysteinyl FMN. The positively charged guanidino group of Arg-222 is thought to stabilize negative charge as it develops at the N1 position of the flavin during flavinylation of the enzyme. Three mutant trimethylamine dehydrogenases were constructed to alter the nature of...

OBJECTIVES The objectives of the study were to compare the different doses of clove oil, Propiscin, and tricaine methane sulphonate (MS 222) in relation to water temperature in pikeperch aquaculture. DESIGN For assessment of this experiment 168 fish (10.77 ± 0.59 cm total body length and 7.88 ± 1.74 g body weight) were used. Three different anaesthetic treatments (Propiscin, clove oil and MS ...

Heisler (1984, 1986) suggested that fish have a maximum extracellular bicarbonate concentration of about 30mmoll~, which can be attained and used for the compensation of blood acidosis induced during environmental hypercapnia. In contrast, Borjeson (1977) and Dimberg & Hoglund (1987) reported HCO3~ concentrations, analysed using Astrup's tonometric method (Astrup, 1956), of 40—SOmmoll" in venou...

The lack of success in predicting the recruitment of fish may to some extent arise from insufficient knowledge of processes affecting egg and larval survival (Chambers and Trippel, 1997). In this context, the quality of eggs and larvae [cf. definitions given in Brooks et al. (Brooks et al., 1997)] and their capacity to develop properly are often mentioned as important factors along with the cor...

BACKGROUND Zebrafish are increasingly used to study the influences of gene mutation and manipulation on cardiac development, structure and function. In this study, a video edge detection system was used to characterise, continuously, cardiac ventricle function in 2-5 days old zebrafish embryos embedded in 0.6% agar and examined under light microscopy at room temperature (22 degrees C). Using vi...

Zebrafish are becoming one of the most used vertebrates in developmental and biomedical research. Fish are commonly killed at the end of an experiment with an overdose of tricaine methanesulfonate (TMS, also known as MS-222), but to date little research has assessed if exposure to this or other agents qualifies as euthanasia (i.e. a "good death"). Alternative agents include metomidate hydrochlo...

The zebrafish has become an important in vivo model in biomedical research. Effective methods must be developed and utilized to deliver compounds or agents in solutions for scientific research. Current methods for administering compounds orally to adult zebrafish are inaccurate due to variability in voluntary consumption by the fish. A gavage procedure was developed to deliver precise quantitie...