Manilkara zapota (L.) P. Royen, a widely adapted and popular tree meant for its appetizing fruits in tropics with no genomic resources like microsatellite markers. In order to develop markers primarily Sapota, the study sequenced partial DNA using next generation sequencing technology on Illumina HiSeq 2500 platform. 3.3 Gb data assembled into 6,396,224 contigs were analysed. From these contigs...

Background: Colorectal cancer (CRC) is the third most common cancer worldwide. microsatellite instability (MSI) is a molecular marker of a deficient mismatch repair system and happens in almost 15% of CRCs. Because of a wide frequency of MSI+ CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detecti...

Papaya (Carica papaya L.) is one of the most economically, medicinally and nutritionally important tropical fruit crops. Expressed sequence tags (ESTs) derived simple sequence repeat (SSR) markers are more valuable as they are derived from conserved genic portion. Development of EST-SSRs markers through in silico approach is cheaper, less time consuming and labour-intensive. In this study, we a...

Leukocytosis is a para neo plastic syndrome which arises nearly in 30% of solid tumor. Anemia, leukocytosis, thrombocytosis and other para neo plastic disorders have been seen with numerous malignancies especially in advanced stages [1]. Para neo plastic syndrome often is seen with gynecologic, lung, breast and hematologic malignancies. Recent studies on leukocytosis and other blood markers in ...

Microsatellite loci were screened from the genomic data of Dysomma anguillare and their composition distribution analysed by bioinformatics for first time. The results showed that 4,060,742 scaffolds with a total length 1,562 Mb obtained high-throughput sequencing 1,160,104 microsatellite MISA screening, which distributed on 770,294 scaffolds. occurrence frequency relative abundance 28.57% 743/...

Microsatellite (simple sequence repeats, SSRs) marker is one of the most widely used markers in marker-assisted breeding. As one type of functional markers, MicroRNA-based SSR (miRNA-SSR) markers have been exploited mainly in animals, but the development and characterization of miRNA-SSR markers in plants are still limited. In the present study, miRNA-SSR markers for Medicago truncatula (M. tru...

OBJECTIVE : Germline mutations in mismatch repair (MMR) genes cause Lynch syndrome (LS). LS is an inherited disease, and an important consequence of MMR deficiency is microsatellite instability (MSI) phenotype. MSI phenotype influences the efficacy of 5 fluorouracil (5-FU) chemotherapy. Reproducible, cost effective, and easy to perform laboratory tests are required to include MSI detection in r...