The solubilization of 80% of the acetylcholinesterase activity of mouse brain was performed by repeated 2h incubations of homogenates at 37 degrees C in an aqueous medium. Analysis of the soluble extract by gel filtration on Sephadex G-200 showed that up to 80% of the enzyme activity was eluted in a peak which was estimated to consist of molecules of about 74000mol.wt. This peak was called the ...

This study analyse the type of release kinetic specific monomers from dental resin composites containing various fluoride-doped calcium phosphates. The behavior urethane dimethacrylate (UDMA), ethoxylated bisphenol-A (bis-EMA) and 1.6-hexanediol ethoxylate diacrylate (HEDA) was evaluated over a period 35 days. Two tailored phosphates doped with different concentrations fluoride salts (VS10% VS2...

A new ruthenium ion imprinted polymer was prepared from the Ru(III) 2-thiobarbituric acid complex (the template), methacrylic acid or acrylamide (the functional monomers), and ethylene glycol dimethacrylate (the cross-linking agent) using 2,2'-azobisisobutyronitrile as the radical initiator. The ion imprinted polymer was characterized and used as a selective sorbent for the solid phase extracti...

Before delivery to endosomes, portions of proCD (procathepsin D) and proSAP (prosaposin) are assembled into complexes. We demonstrate that such complexes are also present in secretions of cultured cells. To study the formation and properties of the complexes, we purified proCD and proSAP from culture media of Spodoptera frugiperda cells that were infected with baculoviruses bearing the respecti...

plastic adhesives, normal butyl cyanoacrylate monomer, isobutyl cyanoacrylate monomer and methyl 2 _ cyaooacrylate monomer, have been utilized in a comparative study with 5-{) silk suture in the repair of transected carotid arteries. follow _ up arteriog rams indicate isobutyl cyanoacrylate monomer and normal butyl cyanoacrylate monomer as having the most impressive rerults with only two thromb...

In obese women (a = 16) at their usual weight, fasting adipose tissue lipoprotein lipase (LPL) activity, obtained by elution with serum and heparin at 40 and 370C, was inversely correlated to plasma estradiol levels (r = -0.724; P = 0.002) and (r = -0.641; P = 0.010), respectively. Furthermore, fasting postheparin plasma LPL activity during a heparin infusion, showed an even stronger inverse co...

Thermodynamic studies on protein adsorption onto chromatographic surfaces mainly focus the molecular level interaction between proteins and ligands. Yet, not much attention is given to study of polymer grafted ligand architecture effect thermodynamic parameters, nor relation parameters directly obtained parameters. These relations are needed in order confer meaning ease future data interpretati...