نتایج جستجو برای: monolayer cell culture

تعداد نتایج: 1881478  

A.R. Nikoofar, B. Goliaei, Gh.R. Fazeli, S. Khoei,

Background: Several cell lines when cultured under proper condition can form three dimensional structures called multicellular tumor spheroids. Tumor spheroids are valuable in vitro models for studying physical and biological behavior of real tumors. A number of previous studies using a variety of techniques have shown no relationship between radiosensitivity and DNA strand breaks in monolayer ...

Journal: :International journal of oncology 2010
Ryoji Eguchi Yoshihiro Fujimori Toshiro Ohta Kazuhiro Kunimasa Takashi Nakano

To study endothelial injury in vitro, we established a three-dimensional (3-D) blood vessel model in which human umbilical vein endothelial cells were grown in the presence of basic fibroblast growth factor and vascular endothelial growth factor. We then performed comparative studies on cisplatin (cis-platinum-diammine-dichloride, CDDP)-induced endothelial injury in 3-D and monolayer cultures. ...

Jila Rajaee Marzieh Ebrahimi Samideh Khoei Seied Rabi Mahdavi,

Introduction: CSCs have been identified in prostate cancer (PCa), one of the most diagnosed malignancies in men over the world, for which radiation resistance is a major problem in the treatment of advanced stages. Cancer stem cells (CSCs) have the ability to self-renew and differentiate to give rise to heterogeneous phenotype of the tumor cells. It is believed that CSCs are ...

2017
Jiaojiao Zhou Jimmy Su Xiaotong Fu Lei Zheng Zhizhong Yin

BACKGROUND Traditional two-dimensional (2-D) monolayer cell culture is vastly different from in vivo physiological conditions, which can lead to inaccurate or insufficient data in areas where response and efficacy within humans are being investigated, such as drug discovery, pathology studies, etc. Misleading results arise from two main disadvantages of monolayer cell culture. First, after seve...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2009
Beate C Litzenburger Hyun-Jung Kim Isere Kuiatse Joan M Carboni Ricardo M Attar Marco M Gottardis Craig R Fairchild Adrian V Lee

PURPOSE This study aimed to test the ability of a new insulin-like growth factor receptor (IGF-IR) tyrosine kinase inhibitor, BMS-536924, to reverse the ability of constitutively active IGF-IR (CD8-IGF-IR) to transform MCF10A cells, and to examine the effect of the inhibitor on a range of human breast cancer cell lines. EXPERIMENTAL DESIGN CD8-IGF-IR-MCF10A cells were grown in monolayer cultu...

ژورنال: Anatomical Sciences Journal 2006
Baghban Eslami Nezhad, Mohammad Reza, Gharezi, Ahmad, Taghi Yar, Leyla,

Purpose: The purpose of this study was to isolate and purify fibroblastic cells from NMRI mouse bone marrow and evaluate their potential in differentiating among condrocytic cell lineage in vitro. Materials and Methods: The cells from NMRI bone marrow were harvested and plated at about 500 cells per well of 6-well plates for several days. The pure fibroblastic cells were not appeared until the...

A.R. Nikoofar, B. Goliaei, M.S. Nezamtaheri, S. Khoei,

Background: Radiotherapy is an established therapeutic modality for prostate cancer. Resveratrol, a natural antioxidant, has been shown to inhibit carcinogenesis and to block the process of tumor initiation and progression. No data is available on the response of cellular spheroid to Reseveratol. In this study we have examined the effect of Resveratol on the radiation response of human p...

Journal: :Spine 2001
J Y Wang A E Baer V B Kraus L A Setton

STUDY DESIGN The mRNA levels of aggrecan and collagen were quantified in intervertebral disc cells cultured under three conditions: primary alginate culture, monolayer culture, and re-encapsulation in alginate after monolayer culture. OBJECTIVES To compare the phenotype of intervertebral disc cells under different culture conditions and to investigate the reversibility of cell phenotype after...

Elliot Elson, Francesca Mascarenhas, Golrokh Malihi,

Previous studies indicate that organ fibroblasts play an important role in wound healing, collagen production, remodeling processes and pathogenesis of progressive heart, lung, renal and hepatic fibrotic diseases. Several studies suggest a possible inhibitory role for adenosine in the regulation of fibroblast proliferation. The effect of adenosine A2 agonists on proliferation and differentiatio...

Journal: :European cells & materials 2010
K Jähn R G Richards C W Archer M J Stoddart

In vitro monolayer culture of human primary osteoblasts (hOBs) often shows unsatisfactory results for extracellular matrix deposition, maturation and calcification. Nevertheless, monolayer culture is still the method of choice for in vitro differentiation of primary osteoblasts. We believe that the delay in mature ECM production by the monolayer cultured osteoblasts is determined by their state...

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