seven stilbenes and one catechin were bioactivity-guidedly isolated from the rhizomes of rheum palmatem. their structures were identified as piceatannol(1), resveratrol(2), piceid(3), rhapontigenin(4), piceatannol-3'-o-β-d-glucopyranoside(5), rhaponticin(6), catechin(7) and desoxyrhapontigenin(8). anti-monoamine oxidase (mao) activities of compounds 1–8 were tested. compounds 1 and 8 showe...

to develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the hplc method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(trifluoromethyl) benzylamine (11) was proved to be an exc...

A simple, sensitive Spectrophotometric determination of monoamine oxidase activity from tissue homogenates is described. This method is based on the high extinction coefficient of 4-hydroxy-3-methoxy-benzaldehyde (vanillin) at 345 nm at pH ~ 11.7. Vanillin is formed by inonoamine oxidase when 4-hydroxy-3-methoxy-benzylamine is used as substrate. The primary oxidation product, 4-hydroxy-3-methox...

The endogenous reactive oxygen species ("ROS") formation is associated with many pathologic states such as inflammatory diseases, neurodegenerative diseases, brain and heart ischemic injuries, cancer, and aging. The purpose of this study was to investigate the endogenous sources for "ROS" formation in intact isolated rat hepatocytes, in particular, peroxisomal oxidases, monoamine oxidase, xanth...

adjuvant; a second intramuscular injection of an equal amount of antigen in Freund’s incomplete adjuvant was given 1 month later. Then 1 week later rabbits were bled, and a crude immunoglobulin preparation was made by the method of Kekwick (1940). The results shown in Fig. 1 suggest that the human neuroblastoma line contains monoamine oxidase activity of an A type, whereas that of MC63 cells is...

Abstract In the present study, eleven compounds (1-11) including nine alkaloids (1-9), one triterpenoid saponin (10) and one formamide (11) were isolated from Talaromyces sp. LGT-2, an endophytic fungus from Tripterygium wilfordi. Their structures were determined based on NMR and ESI-MS spectral data, as well as comparing with previous literature data. This is the first report of the isolation ...

Abstract In the present study, eleven compounds (1-11) including nine alkaloids (1-9), one triterpenoid saponin (10) and one formamide (11) were isolated from Talaromyces sp. LGT-2, an endophytic fungus from Tripterygium wilfordi. Their structures were determined based on NMR and ESI-MS spectral data, as well as comparing with previous literature data. This is the first report of the isolation ...

IT HAS previously been demonstrated that the monoamine oxidase inhibitor, RO 41038 (DL-serine-N2-isopropylhydrazide) is a clinically useful drug in the treatment of arterial hypertension." 2 Furthermore, all the compounds that inhibit monoamine oxidase in vivo are hypotensive in man to a greater or lesser degree.3 Although the precise manner in which the disturbances of catecholamine metabolism...

The pressor responses to oral and intravenous tyramine were not different from controls in migrainous patients with or without a history of attacks triggered by foods. However, patients who reported a dietary trigger were more likely to develop headache after tyramine administration than those without such a dietary history. Pressor responses to intravenous tyramine in patients with cluster hea...