We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein.

In the educational context it is examined the teacher-stress association. The teacher’s stress is related with variables of academic environment, of out of the school and of the teacher. The impact of that association can be see in the teacher’s health, in his achievement and in the quality of the teaching-learning process. With the objective to present one view of how issue is being considered...

We demonstrate that an oft-used indirect attitude assessment technique—the attitude activation paradigm—accurately assesses attitudes only when participants attend to the prime stimuli during the attitude activation task. Attitude activation attitudes toward obviously valenced words (e.g., torture, liberty) were more sensitive to attitude valence and extremity when participants were required to...

Ascertaining the relationships between sediment transport processes and vegetation cover is essential for watershed soil and water conservation. However, it is not easy to realize this target on a large scale. In this study, the location-weighted landscape contrast index (LCI) based on the “source-sink” theory of ecological processes was introduced to unravel the response of sediment yield to v...

Historically photochemical mechanisms and air quality models have been evaluated primarily in terms of there ability to simulate observed O3 data. There is an increasing awareness that mechanism and models must also be evaluated in terms of their ability to simulate the fundamental chemical processes that control O3 formation and the sensitivity of O3 to emissions reductions (Arnold et al., 199...

Short indicator RNA sequences (<100 bp) persist after autoclaving and are recovered intact by molecular amplification. Primers targeting longer sequences are most likely to produce false positives due to amplification errors easily verified by melting curves analyses. If short indicator RNA sequences are used for virus identification and quantification then post autoclave RNA degradation method...

This paper presents some aspects related to the issues of aggregating economic indicators. Departing from the research of Gh. Paun (1983) we will prove a theorem which states that under certain, natural assumptions, it is impossible to obtain an optimum aggregation. Unlike the original work of Paun, in this paper we are giving a rather simple proof of this theorem.

The validity of the Michaelis-Menten-Briggs-Haldane approximation for single enzyme reactions has recently been improved by the formalism of the total quasi-steady-state approximation. This approach is here extended to fully competitive systems, and a criterion for its validity is provided. We show that it extends the Michaelis-Menten-Briggs-Haldane approximation for such systems for a wide ran...