CRISPR/dCas9-based labeling has allowed direct visualization of genomic regions in living cells. However, poor labeling efficiency and signal-to-background ratio have limited its application to visualize genome organization using super-resolution microscopy. We developed (Po)STAC (Polycistronic SunTAg modified CRISPR) by combining CRISPR/dCas9 with SunTag labeling and polycistronic vectors. (Po...

Combined use of in situ passivation and macrophytes is a valuable technology that exerts remarkable effects on aquatic systems. However, the effectiveness ecological functions this combined for macrophytes-dominated eutrophic (MDE) lakes with organophosphorus-controlled internal phosphorus (P) loading were poorly understood. In study, simulation experiments performed to study combination La-mod...

This research study aims to investigate the feasibility of incorporating high-density polyethylene waste (HDPEW) into bitumen applications. Two conventional conditions bitumen, namely, aged (AB) and virgin (VB), are rejuvenated modified, respectively, using post-consumer HDPEW sourced out bottle crates. The outcome (Pyro oil, PO-HDPEW) pyrolysis thermochemical process is used by 10, 20, 30% rej...

Trout testis maturation is characterized by the complete replacement of the histones by the protamines. During this process, the protamines are extensively modified by enzymatic phosphorylation of their seryl residues. In addition, a methionyl residue is transiently incorporated at the NH2 terminus of newly synthesized protamine. However, these modifying groups are removed by the time that sper...

In this paper, a novel method of partial coating to reduce the Radar Cross Section (RCS) of a complex shaped perfectly electrically conducting (PEC) aircraft, of electrically large size, is proposed. The physical optics (PO) method is used to identify the hot spots of the PEC aircraft. An empirical procedure is then proposed to coat partial contiguous areas with Radar Absorbent Material (RAM). ...

Locally partial-ordered spaces (local po-spaces) have been used to model concurrent systems. We provide equivalences for these spaces by constructing a model category containing the category of local po-spaces. We show the category of simplicial presheaves on local po-spaces can be given Jardine's model structure, in which we identify the weak equivalences between local po-spaces. In the proces...

A set of new polymers that can be used as phase forming components in aqueous two-phase systems is presented. All polymers studied have thermoseparating properties i.e. form one separate polymer enriched phase and one aqueous solution when heated above the critical temperature. This property makes the polymers attractive alternatives to the polymers used in traditional aqueous two-phase systems...

Natural phosphodiester bond CpG-DNA that contains immunomodulatory CpG motifs (PO-DNA) upregulates the expression of proinflammatory cytokines and induces an Ag-driven Th1 response in a CG sequence-dependent manner in mice. In humans, only phosphorothioate backbone-modified CpG-DNA (PS-DNA) and not PO-DNA has immunomodulatory activity. In this study, we found that liposome-encapsulated PO-DNA u...

Reverse transcription is one step of the retroviral development which can be inhibited by antisense oligonucleotides complementary to the RNA template. 2'-O-Alkyl oligoribonucleotides are of interest due to their nuclease resistance, and to the high stability of the hybrids they form with RNA. Oligonucleotides, either fully or partly modified with 2'-O-alkyl residues, were targeted to an RNA te...

Dual-layer and tri-layer broadband antireflective (AR) films with excellent transmittance were successfully fabricated using base-/acid-catalyzed mixed sols and propylene oxide (PO) modified silica sols. The sols and films were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), transmission electron microscope (...