The aim of this research was to develop reliable, easy-to-perform and cheap method for measuring protein oxidation in complex samples such as aquaculture feed within various sources. For that purpose modified 2,4-dinitrophenylhydrazyne (DNPH)-based quantification carbonyls employed, while the modification consisted using different solutions extraction, time extraction concentration trichloracet...

The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein and absolute concentrations cannot be obtained. The procedure of Lowry et al. (1) is no exception, but its sensitivity is moderately constant from protein to protein, and it has been so widely use...

As part of a continuing study of the quantitative distribution of biochemically significant substances and activities in the separate zones of the adrenal gland in various functional states, the present report deals with pyridine nucleotidrs. In addition to other coenzyme functions, the pyridine nucleotides are involved in the hydroxylation of steroids in the adrenal (1, 2). Total osidized and ...

background: the most reliable indicator of vitamin d status is circulating concentration of 25‑hydroxycalciferol (25(oh) d) routinely determined by enzyme‑immunoassays (eia) methods. this study was performed to compare commonly used competitive protein‑binding assays (cpba)‑based eia with the gold standard, high‑pressure liquid chromatography (hplc). methods: concentrations of 25(oh) d in sera ...

We developed a sensitive fluorescence assay for the quantitation of proteins in solution using the NanoOrange reagent, a merocyanine dye that produces a large increase in fluorescence quantum yield upon interaction with detergent-coated proteins. The NanoOrange assay allowed for the detection of 10 ng/mL to 10 micrograms/mL protein with a standard fluorometer, offering a broad, dynamic quantita...

Some recent modifications of the protein assay by the method of Lowry, Rosebrough, Farr, and Randall (1951, .I. Biol. Chem. 193, 265-275) have been reexamined and altered to provide a consolidated method which is simple, rapid, objective, and more generally applicable. A DOC-TCA protein precipitation technique provides for rapid quantitative recovery of soluble and membrane proteins from interf...

Coffin-Lowry syndrome (CLS) is an X-linked disorder, which affects hemizygous males more severely than females. It is characterized by mental retardation, short stature, head and facial abnormalities, skeletal anomalies and developmental delays. The signs and symptoms vary in different people. We report a 14-year-old male patient, diagnosed with CLS based on his clinical features. Genetic testi...