We propose a novel method and software tool, Strawberry, for transcript reconstruction and quantification from RNA-Seq data under the guidance of genome alignment and independent of gene annotation. Strawberry consists of two modules: assembly and quantification. The novelty of Strawberry is that the two modules use different optimization frameworks but utilize the same data graph structure, wh...

The acute effect of complexity in aroma composition on perceived satiation and food intake was investigated in 41 young, healthy, and normal weight subjects. Subjects consumed 2 different strawberry-aromatized sweetened yogurt products (i.e., test and placebo product) in either an olfactometer-aided or an ad libitum eating experimental design. The test product was aromatized with a multicompone...

Background and Aims: Influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. A variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. Methods: Nasal and pharyngeal samples taken from patients were inoculated into Madin-Darby canine kidney (MOCK) cells and embryonated chicken eggs (ECEs). The culture media was a...

ABSTRACT Anthracnose is one of the major fungal diseases of strawberry occurring worldwide. In Israel, the disease is caused primarily by the species Colletotrichum acutatum. The pathogen causes black spot on fruit, root necrosis, and crown rot resulting in mortality of transplants in the field. The host range and specificity of C. acutatum from strawberry was examined on pepper, eggplant, toma...

BACKGROUND: Information on absorption and metabolism of strawberry polyphenols is limited. OBJECTIVE: The aim of this study was to characterize and quantify plasma polyphenols/anthocyanins across 4 doses of strawberry using Q-TOF LC/MS and LC-MS/MS analysis. METHOD: Plasma was collected from 5 subjects (n= 5) every 30–60 min for 6 h after consuming beverages containing 0, 10, 20 or 40 g freeze-...

ABSTRACT Ninety-five isolates of Colletotrichum including 81 isolates of C. acutatum (62 from strawberry) and 14 isolates of C. gloeosporioides (13 from strawberry) were characterized by various molecular methods and pathogenicity tests. Results based on random amplified polymorphic DNA (RAPD) polymorphism and internal transcribed spacer (ITS) 2 sequence data provided clear genetic evidence of ...

Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine...

Xanthomonas fragariae is a foliar pathogen of strawberry that is of significant concern to nursery production of strawberry transplants and field production of strawberry fruit. Long-read sequencing was employed to generate finished genomes for two isolates (each with one chromosome and two plasmids) from symptomatic plants in northern California.

A method was developed for evaluating strawberry susceptibility to Verticillium wilt in in vitro conditions. Micropropagated strawberry shoots of 15 strawberry cultigens, differing in susceptibility to the disease in field conditions, were treated in vitro with fungal homogenate of Verticillium dahliae, diluted with sterile water in a proportion of 1:10 in order to decrease the pathogen’s press...

A comprehensive in vitro study involving antiglycation, antioxidant and anti-diabetic assays was carried out in mature fruits of strawberry. The effect of aqueous extract of mature strawberry fruits on glycation of guanosine with glucose and fructose with or without oxidizing entities like reactive oxygen species was analyzed. Spectral studies showed that glycation and/or fructation of guanosin...