Graft-versus-host disease is a severe complication of allogeneic stem cell transplantation. The major role is played by alloreactive donor T-cell clones leading to host tissue damage. Selective depletion is a strategy to eliminate host-reactive donor T cells from haematopoietic stem cell allografts to prevent graft-versus-host disease while conserving useful donor immune functions. We have used...

Donor leukocytes in therapeutic blood components are implicated in transfusion-related complications ranging from alloimmunization to graft-versus-host disease (GVHD) to viral transmission and reactivation. To further characterize the kinetics of donor leukocyte clearance after allogeneic transfusion, we developed allele-specific polymerase chain reaction (PCR) assays directed at a single-copy ...

Background: Dendritic cells (DC) induce tumor or pathogen-specific T cell responses in humans. Several laboratories have developed culture systems, including maturation factors for human DC from peripheral blood monocytes. We comprehensively compared standard maturation stimulus, an autologous monocyte-conditioned medium (MCM), with heparin for their ability to promote uniformly mature DC that ...

The disparity index is an expression of histocompatibility difference between two siblings with identical human leukocyte antigen (HL-A) type who are nonreactive in mixed leukocyte culture (MLC) test. This index is derived from a third-party MLC test. The disparity index was found to be correlated with the severity of graft-versus-host reaction in bone marrow transplantation between HL-A-identi...

background: unrestricted somatic stem cells (ussc) are cord blood stem cells that have been considered as candidates for the regulation of immune responses. therefore, potential exists for their use in the suppression of immune response after transplantation surgery. objective: the aim of this study was evaluation of the effect of ussc on mixed lymphocyte reaction (mlr) as a model for graft rej...

background: dendritic cells (dc) induce tumor or pathogen-specific t cell responses in humans. several laboratories have developed culture systems, including maturation factors for human dc from peripheral blood monocytes. we comprehensively compared standard maturation stimulus, an autologous monocyte-conditioned medium (mcm), with heparin for their ability to promote uniformly mature dc that ...