To investigate the role of circ_0084927 in lung cancer cell sensitivity to cisplatin. Half-maximal inhibitory concentration value cisplatin was detected by counting kit-8. and microRNA-634 expression were determined reverse transcription quantitative polymerase chain reaction. Cell kit-8, scratch healing, colony formation as well Transwell experiments used for analyzing viability, healing rate,...

in the retinoblastoma research, it is of great interest to identify molecular markers associated with the genetics of tumorigenesis. micrornas (mirnas) are small non-coding rna molecules that play a regulatory role in many crucial cellular pathways such as differentiation, cell cycle progression, and apoptosis. a body of evidences showed dysregulation of mirnas in tumor biology and many disease...

objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

To investigate the mechanism of lidocaine in inhibiting proliferation, migration and invasion breast cancer cells. Human cell line Michigan Cancer Foundation-7 cells were treated with lidocaine. Cell proliferation was detected by 2,5-diphenyl-2H-tetrazolium bromide, Transwell, microRNA-495-3p expression quantitative reverse transcription polymerase chain reaction fibroblast growth factor 9, cyc...

The enzyme 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) is selectively expressed in aldosterone target tissues, conferring aldosterone selectivity for the mineralocorticoid receptor. A diminished activity causes salt-sensitive hypertension. The mechanism of the variable and distinct 11β-hydroxysteroid dehydrogenase type 2 gene (HSD11B2) expression in the cortical collecting duct is poorly...

To explore the function of kaempferol on lung cancer cell A549 and microRNA-199 variation is objective study. The proliferation was detected by counting kit-8. expression mammalian target rapamycin, phosphorylated light chain 3-?/?, beclin-1, B-cell lymphoma 2 2-associated X protein showed Western blot. determined quantitative polymerase reaction. adenocarcinomic human alveolar basal epithelial...

mirs are 17-25 nucleotide non-coding rnas. these rnas target approximately 80% of protein coding mrnas. mirs control gene expression and altered expression of them affects the development of cancer. mirs can function as tumor suppressor via down-regulation of proto-oncogenes and may function as oncogenes by suppressing tumor suppressors. myeloproliferative neoplasias (formerly known as chronic ...