1. Besides miR-133a, miR-133b and miR-208a, there are several other muscle-specific miRNAs, such as miR-1, etc. Why only these miRNAs were used in this study? 2. P2: qRT-PCR is not quantitative real-time polymerase chain reaction, but quantitative reverse transcription-polymerase chain reaction. 3. P6: “To date, no housekeeping miRNAs have been established and validated to normalize for the miR...

abstract        background and objective: various cellular factors affect the process of hiv activity. one of these cellular factors are structures known as microrn that are expected to be involved in controlling hiv replication and infectivity. the expression of one or a set of them may represent the patient's clinical conditions. in this study,  the expression of mir-29a and mir-29b invo...

BACKGROUND Leukemia seriously threatens human life and health. MicroRNAs can regulate cell growth, proliferation, and death. This article investigated the role of miR-29 on regulating leukemia cell growth, proliferation, and apoptosis. MATERIAL AND METHODS miR-29 and scramble miRNA were transfected to K562 cells. MTT assay, colony formation assay, caspase-3 activity detection, and flow cytome...

MicroRNA-29a (miR-29a) is a well characterized fibro-inflammatory molecule and its aberrant expression linked to variety of pathological liver conditions. The long-term effects high-fat diet (HFD) in combination with different levels EtOH consumption on miR-29a pathobiology are unknown. Mice at 8 weeks age were divided into five groups (calorie-matched plus water (CMD) as control group, HFD dis...

MicroRNAs (miRNAs) have emerged as essential gene expression regulators associated with human diseases such as colorectal cancer (CRC). The purpose of this study was to evaluate the expression of miR-330-3p and its target gene BMI1 in tissue samples of patients with CRC, polyp, and healthy adjacent tissue samples and their association with clinicopathological and demographic factors such as age...

We introduce aligned hierarchies, a low-dimensional representation for music-based data streams, such as recordings of songs or digitized representations of scores. The aligned hierarchies encode all hierarchical decompositions of repeated elements from a high-dimensional and noisy music-based data stream into one object. These aligned hierarchies can be embedded into a classification space wit...

It is unknown whether fibrosis-associated microRNAs: miR-21, miR-26, miR-29, miR-30 and miR-133a are linked to cardiovascular (CV) outcome. The study evaluated the levels of extracellular matrix (ECM) fibrosis and the prevalence of particular microRNAs in patients with dilated cardiomyopathy (DCM) to investigate any correlation with CV events. METHODS Seventy DCM patients (48 ± 12 years, EF 2...

We aimed at assessing the stability of candidate reference genes in urine sediments of men subjected to digital rectal examination for suspected prostate cancer (PCa). Two microRNAs (miR-191 and miR-25) and 1 small nucleolar RNA (SNORD48) were assayed in 35 post-DRE urine sediments of men with PCa and in 26 subjects with histologically confirmed benign prostatic hyperplasia (BPH). The stability...

Dorsal root ganglia (DRG) neurons spontaneously undergo neurite growth after nerve injury. MicroRNAs (miRNAs), as small, non-coding RNAs, negatively regulate gene expression in a variety of biological processes. The roles of miRNAs in the regulation of responses of DRG neurons to injury stimuli, however, are not fully understood. Here, microarray analysis was performed to profile the miRNAs in ...