Thrombus lysis is the consequence of a restricted number of reactions localized on the surface of fibrin and cell membranes. A functional defect or an insufficient fibrinolytic response may lead to thrombosis with severe or fatal clinical consequences. Despite this clinical need, and real progress in the knowledge of the different components of this system (plasminogen activators, inhibitors an...

Identification and quantitation of cellular proteins associated with HIV-1 particles are complicated by the presence of nonvirion-associated cellular proteins that copurify with virions. Many cellular proteins are associated with nonviral particles that bud from the surface of cells called microvesicles. Microvesicles band in sucrose gradients in a range of densities that includes the same dens...

Coated microvesicles isolated from bovine neurohypophyses could be loaded with Ca2+ in two different ways, either by incubation in the presence of ATP or by imposition of an outwardly directed Na+ gradient. Na+, but not K+, was able to release Ca2+ accumulated by the coated microvesicles. These results suggest the existence of an ATP-dependent Ca2+-transport system as well as of a Na+/Ca2+ carr...

Administration of mesenchymal stem cells (MSCs) improves the recovery from acute kidney injury (AKI). The mechanism may involve paracrine factors promoting proliferation of surviving intrinsic epithelial cells, but these factors remain unknown. In the current study, we found that microvesicles derived from human bone marrow MSCs stimulated proliferation in vitro and conferred resistance of tubu...

Microvesicles (also called microparticles) have become a hot topic recently and transport proteins, mRNA, and microRNA.1–3 They have been proposed to play roles in numerous processes, including coagulation, inflammation, immune response, cell activation, and cancer.1–3 Microvesicles are small (0.1–1 m) membrane vesicles that are released from activated and apoptotic cells. They contain proteins...

Extracellular vesicles (microvesicles), such as exosomes and shed microvesicles, contain a variety of molecules including proteins, lipids, and nucleic acids. Microvesicles appear mostly to originate from multivesicular bodies or to bud from the plasma membrane. Here, we review the convergence of microvesicle biogenesis and aspects of viral assembly and release pathways. Herpesviruses and retro...

Ischemia-driven islet isolation procedure is one of the limiting causes pancreatic transplantation. Ischemia-reperfusion process associated with endothelium dysfunction and release pro-senescent microvesicles. We investigated whether endothelial microvesicles prompt senescence in vitro. Pancreatic islets were isolated from male young rats. Replicative was induced by serial passaging primary por...

Microvesicles (diameter ca 200 nm) from the cell-free supernatant of U87MG human glioblastoma cell caused platelet aggregation and coagulation in a manner identical with that previously shown for the intact cells. Both activities were inhibited by dansylarginine -N-(3-ethyl-1,5-pentanediyl) amide (DAPA), confirming the thrombin-dependent nature of both activities. The specific activities per mi...

Aims. To investigate how circulating microvesicle phenotypes correlate with insulin sensitivity, body composition, plasma lipids, and hepatic fat accumulation. We hypothesized that changes elicited by testosterone replacement therapy are reflected in levels of microvesicles. Methods. Thirty-nine type 2 diabetic males with lowered testosterone levels were assigned to either testosterone replacem...

The transbilayer movement of phosphatidylserine from the inner to the outer leaflet of the membrane bilayer during platelet activation is associated with the release of procoagulant phosphatidylserine-rich small membrane vesicles called platelet-derived microvesicles. We tested the effect of lactadherin, which promotes the phagocytosis of phosphatidylserineexpressing lymphocytes and red blood c...