When a microsome fraction is prepared from rat liver by the method of de Duve et al . (11), it contains about 20% of the proteins of the homogenate, 75% of characteristic microsomal hydrolases (such as glucose 6-phosphatase, nucleoside diphosphatase, esterase), almost the same proportion of typical microsomal oxidoreductases (NADPH and NADH: cytochrome c reductases, cytochrome b 5 , aminopyrine...

The purpose of these studies was to determine the properties of the membrane-bound cytidylyltransferase in adult lung and to assess the relationship between the microsomal enzyme and the two forms of cytidylyltransferase in cytosol. Microsomes, isolated by glycerol density centrifugation, contained significantly less cytidylyltransferase than microsomes isolated by differential centrifugation (...

1. The phosphatidylinositol-exchange protein from bovine brain was used to determine to what extent phosphatidylinositol in rat liver microsomal membranes is available for transfer. 2. The microsomal membranes used in the transfer reaction contained either phosphatidyl[2-3H]inositol or 32P-labelled phospholipid. The 32P-labelled microsomal membranes were isolated from rat liver after an intrape...

When a microsome fraction is prepared from rat liver by the method of de Duve et al . (11), it contains about 20% of the proteins of the homogenate, 75% of characteristic microsomal hydrolases (such as glucose 6-phosphatase, nucleoside diphosphatase, esterase), almost the same proportion of typical microsomal oxidoreductases (NADPH and NADH: cytochrome c reductases, cytochrome b 5 , aminopyrine...

Plasma membranes were isolated using the aqueous polymer two-phase partition method from the algae Chara corallina and Chara longifolia, algae which differ in their ability to grow in saline environments. Enrichment of plasma membrane and depletion of tonoplast relative to the microsomal fraction was monitored using phosphohydrolase assays and cross-reactions to antibodies raised against higher...

Rat liver microsomal glycoproteins were purified by affinity chromatography on concanavalin A Sepharose columns from membrane and content fractions, separated from rough microsomes (RM) treated with low concentrations of deoxycholate (DOC). All periodic acid-Schiff (PAS)-positive glycoproteins of RM showed affinity for concanavalin A Sepharose; even after sodium dodecyl sulfate (SDS) acrylamide...

Rat liver microsomal glycoproteins were purified by affinity chromatography on concanavalin A Sepharose columns from membrane and content fractions, separated from rough microsomes (RM) treated with low concentrations of deoxycholate (DOC). All periodic acid-Schiff (PAS)-positive glycoproteins of RM showed affinity for concanavalin A Sepharose; even after sodium dodecyl sulfate (SDS) acrylamide...

Rat liver microsomal glycoproteins were purified by affinity chromatography on concanavalin A Sepharose columns from membrane and content fractions, separated from rough microsomes (RM) treated with low concentrations of deoxycholate (DOC). All periodic acid-Schiff (PAS)-positive glycoproteins of RM showed affinity for concanavalin A Sepharose; even after sodium dodecyl sulfate (SDS) acrylamide...

Purified Signal Recognition Protein (SRP) has previously been shown to be required for the translocation of secretory proteins across the microsomal membrane (Walter and Blobel, 1980 . Proc. Nat/ . Acad. Sci. U . S. A. 77:7,112-7,116) and to function in the early events of this process (Walter and Blobel, 1981. J. Cell Biol . 91 :557-561) . We demonstrate here that the S subunit of acetylcholin...

Treatment of rat liver rough microsomes (3.5 mg of protein/ml) with sublytical concentrations (0.08%) of the neutral detergent Triton X-100 caused a lateral displacement of bound ribosomes and the formation of ribosomal aggregates on the microsomal surface. At slightly higher detergent concentrations (0.12-0.16%) membrane areas bearing ribosomal aggregates invaginated into the microsomal lumen ...