Key Message Agrobacterium tumefaciens was used to transform radiata pine shoots and to efficiently produce stable genetically modified pine plants. Abstract Micropropagated shoot explants from Pinus radiata D. Don were used to produce stable transgenic plants by Agrobacterium tumefaciens–mediated transformation. Using this method any genotype that can be micropropagated could produce stable tra...

the effect of sub culturing frequencies and different cytokinins (benzyl amino purine (bap), 2-isopentenyl adenine (2ip) on genetic stability of micropropagated shoots from olive plant were investigated by using physiological traits and random amplified polymorphic dna (rapd) markers. axillary buds of the olive (cv. dezful) plants were cultured and subcultured in dkw medium, supplemented with b...

A high-frequency, season-independent, in vitro regeneration of Ficusreligiosa was developed, followed by comparative acetylcholinesterase inhibitory (AChEI) activity assay of the in vitro raised and conventionally grown plants. The use of AChEI activity is the most accepted strategy for the treatment of Alzheimer disease. Fully expanded, mature leaves were cut into different segments to initiat...

An in vitro protocol has been established for clonal propagation of Nothapodytes nimmoniana which is an important source of Camptothecin (CPT). Elite source was identified based on the chemical potency to accumulate the optimum level of CPT. Different types and concentrations of plant growth regulators were used to study their effect on inducing multiple shoots from the explants regenerated fro...

micropropagated plantlets derived from three different grape rootstock genotypes namely, dogridge (vitis champini), so4 (v. berlandieri×v. rupestris) and ari-h-144 (v. vinifera×v. labrusca) were subjected to randomly amplified polymorphic dna (rapd) and inter simple sequence repeats (issr) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...

An efficient genetically stable regeneration protocol with increased phytochemical production has been established for Dendrobium nobile, a highly prized orchid for its economic and medicinal importance. Protocorm like bodies (PLBs) were induced from the pseudostem segments using thidiazuron (TDZ; 1.5 mg/l), by-passing the conventional auxin-cytokinin complement approach for plant regeneration....

The use of micropropagated trees has been widely extended, since micropropagation is a convenient technique of vegetative propagation that shows a number of interesting advantages, mainly for woody fruit trees. However, only few studies can be found in the literature on the field performance of micropropagated trees. In this work, the performance of different micropropagated fruit-tree rootstoc...

A study was carried out to identify of different cultivars and monitor somaclonal variations of banana during rapid mass micropropgation using Inter simple sequence repeats (ISSR) marker. DNA templates from 30 Banana cultivars were evaluated using 45 primers. Genetic diversity is analyzed by one statistical procedure: hierarchical classification. Total number of bands varied between the various...

Micropropagated and seed-born silver birches (Betula pendula Roth) were compared for survival, height growth and occurrence of biotic damage (voles, hares, mooses, stem lesions and cankers) in fi eld trials in southern Finland. The material consisted of 11 clones and 10 different lots of seedlings growing in 10 fi eld trials, established in clearcut forest cultivation areas. The plants were 6–7...