PURPOSE In this paper we show that optimization-based autofocus may be used to overcome the instabilities that have, until now, made high-resolution theoretically-exact tomographic reconstruction impractical. To our knowledge, this represents the first successful use of theoretically-exact reconstruction in helical micro computed tomography (micro-CT) imaging. We show that autofocus-corrected, ...

In order to understand and to simulate the functional behaviour of porous structures during loading, quantitative knowledge of the relationship between the morphology and the mechanical behaviour is necessary. Therefore, the porous structures need to be quantified morphologically. In this research, the quantification will be performed by using micro-CT. However, to date, this technique encounte...

Aims In preclinical setting, micro-CT is used to provide anatomical information of small animals (e.g. mice), and can be used in conjunction with functional imaging modalities, which provide information on biological processes in tissues. The combination of micro-CT with functional imaging provides localization of the investigated process, i.e. physiology or disease, within the animal and furth...

In this Letter, we present B-scan-sectioned dynamic micro-optical coherence tomography (BD-MOCT) for high-quality sub-cellular contrast imaging. Dynamic (D-MOCT) is a functional optical (OCT) technique performed on high-resolution (micron level) OCT systems; hundreds of consecutive B-scans need to be acquired signal extraction, which requires relatively long data acquisition time. Bulk motions ...

Multiphoton micro-spectroscopy, employing diffraction optics and electron-multiplying CCD (EMCCD) cameras, is a suitable method for determining protein complex stoichiometry, quaternary structure, and spatial distribution in living cells using Förster resonance energy transfer (FRET) imaging. The method provides highly resolved spectra of molecules or molecular complexes at each image pixel, an...

This paper aims to evaluate the impact of spectral filtration on image quality in a microcomputed tomography (micro-CT) system. A mouse phantom comprising 11 rods for modeling lung, muscle, adipose, and bones was scanned with 17 s and 2 min, respectively. The current (μA) for each scan was adjusted to achieve identical entrance exposure to the phantom, providing a baseline for image quality eva...