Ruminants have high concentrations of peptide-bound amino acids in the circulation. Earlier studies in our laboratory showed that a myogenic cell line (C2C12) developed from mouse skeletal muscle and a mammary epithelial cell line (MAC-T) developed from bovine mammary epithelial tissues are able to utilize peptides as amino acid sources. In this study, primary cultures of ovine myogenic satelli...

a total of 160 day-old male broiler chickens (ross 308) were divided in a completely randomized design experiment, and assigned to 8 treatments and 4 replicates in their experimental units (cages). treatments included: t1: basal diet with no additional methionine, t2: basal diet plus 0.06, t3: basal diet plus 0.11, t4: basal diet plus 0.17 % of dl-methionine, t5: basal diet plus 0.06, t6: basal...

A synthetic medium, consisting of inorganic salts and any of a number of carbon sources, supported the aerobic growth of Paracoccus halodenitrificans when supplemented with thiamine. The same medium plus an appropriate nitrogenous oxide supported anaerobic growth when additionally supplemented with methionine. The observation that vitamin B12 or betaine replaced methionine suggested that P. h...

5'-Deoxy-5'-methylthioadenosine and 5'-deoxy-5'-methylthioinosine, which are metabolized to the methionine precursor, 5-methylthioribose-1-phosphate, by 5'-deoxy-5'-methylthioadenosine phosphorylase and purine nucleoside phosphorylase, respectively, can serve as sources of methionine for cultured HL-60 promyelocytic leukemia cells. CCRF-CEM T-cell leukemia cells, which lack 5'-deoxy-5'-methylth...

To determine the effect of dietary methionine supplementation on intestinal amino acid/peptide/monocarboxylic acid transporter gene expression, a total of 242 male Cobb-500 broilers were fed from day of hatch to 35 d of age using a basal diet deficient in sulfur amino acids (control), or the control diet supplemented with dl-methionine (dl-Met), l-methionine (l-Met) or methionine analog dl-2-hy...

Wiebers, Joyce L. (Purdue University, West Lafayette, Ind.), and Harold R. Garner. Use of S-methylcysteine and cystathionine by methionineless Neurospora mutants. J. Bacteriol. 88:1798-1804. 1964.-Radioactive methionine was found in hydrolysates of various strains of Neurospora crassa when either S-methylcysteine (SMC)-C(14)H(3) or SMC-S(35) is the sole addition to minimal medium. Isotope produ...

The aim of the study was to evaluate the accuracy of ileal amino acid digestibility coefficients in dietary formulation for broiler chickens. Two relatively poor quality protein sources, a meat-and-bone meal and a dried blood meal were used. The meals were subjected to the ileal assay and respective mean coefficients of digestibility of 0.58 and 0.67 were found for the first-limiting amino acid...