The recent increase in gram-negative bacteria coproducing multiple classes of β-lactamases has made it difficult to accurately identify metallo-β-lactamase (MBL) producers. In the present study, six methods for detecting MBL producers were compared using 56 gram-negative bacterial isolates that produce various β-lactamases. Sodium mercaptoacetic acid (SMA) and EDTA were used as inhibitors for a...

Objective: In addition to assessing the phenotypic development of biofilms and beta-lactamases, in current study, Pseudomonas aeruginosa isolates from intensive care units (ICU) patients with prevalent drug resistance are being evaluated. Methods: Standard CLSI recommended protocols were utilized detect beta-lactamases such as extended-spectrum β-lactamases (ESBL), metallo beta-lactamase (MBL),...

BACKGROUND Infectious by Pseudomonas aeruginosa has spread worldwide and metallo-beta-lactamases (MBL) are being reported with increasing frequency. The aim of this study was to investigate the antibiotic susceptibility and distribution of blaVIM and blaIMP genes in P. aeruginosa isolates from Zanjan Province of Iran. METHODS A total of 70 P. aeruginosa isolates were identified from patients ...

Metallo-beta-lactamases (MBLs) are being reported with increasing frequency worldwide. The aim of this study was to investigate the prevalence of blalMP-1, blaVIM-1,2 and blaSPM-1 genes encoding metallo-beta-lactamases (MBLs) among a collection of Pseudomonas aeruginosa strains isolated from patients at different hospitals in Tehran and to trace the disseminated clones at these hospitals by pul...

One strategy employed by bacterial strains to resist beta-lactam antibiotics is the expression of metallo-beta-lactamases requiring Zn(2+) for activity. In the last few years, many new zinc beta-lactamases have been described and several pathogens are now known to synthesize members of this class. Metallo-beta-lactamases are especially worrisome due to: (1) their broad activity profiles that en...

PURPOSE Two Korean nationwide studies showed that metallo-beta-lactamases (MBLs)-producing-Pseudomonas spp. are not rare. The aim of this study was to assess the trends of MBL-producing isolates among imipenem-resistant isolates of Pseudomonas spp. MATERIALS AND METHODS Imipenem-resistant clinical isolates were collected from 23 hospitals and one commercial laboratory participating in the KON...

conclusions: the prevalence of metallo-beta-lactamase-producing a. baumannii strains detected in this study is of great concern and highlights the need of infection control measures including antibacterial management and prompt identification of beta-lactamase-producing isolates. results: the resistance of a. baumannii isolates against tested antibiotics were as follow: 108 (100%) to cefotaxime...

b ackground : the emergence and rapid spread of metallo-beta-lactamase (mbl) producing acinetobacter spp. are of great concern worldwide due to limited treatment options. epidemiologic studies of the causing genes are important for prevention. methods :   in  this  study,  70  imipenem-resistant  acinetobacter  strains  were isolated from health care associated infections. these isolates were s...

Klebsiella pneumoniae is a pathogen primarily capable of causing urinary tract infections and pneumonia in otherwise healthy people. Most of the strains are frequently resistant to numerous antibiotics, responsible for conferring multidrug resistance. This study was conducted to determine the prevalence of extended spectrum β-lactamases (ESBL), AmpC β-lactamases and metallo β-lactamases (MBL) a...