Various inhibitors of metallo-beta-lactamases have been reported; however, none are effective for all subgroups. Those that have been found to inhibit the enzymes of subclass B2 (catalytically active with one zinc) either contain a thiol (and show less inhibition towards this subgroup than towards the dizinc members of B1 and B3) or are inactivators behaving as substrates for the dizinc family ...

Multidrug resistance has been increasing among Gram-negative bacteria and is strongly associated with the production of both chromosomal- and plasmid-encoded beta-lactamases, whose number now exceeds 890. Many of the newer enzymes exhibit broad-spectrum hydrolytic activity against most classes of beta-lactams. The most important plasmid-encoded beta-lactamases include (a) AmpC cephalosporinases...

A series of mercaptoacetic acid thiol esters have been identified as metallo-beta-lactamase inhibitors. Electrospray mass spectrometry (ESMS) has shown that irreversible inhibition of the Bacillus cereus II metallo-beta-lactamase by SB214751, SB214752, and SB213079 was concomitant with a 90-Da increase in mass of the enzyme. Tryptic digestion of the B. cereus II inhibited with SB214751 illustra...

The mechanisms responsible for reduced susceptibility to imipenem (MIC 2-16 mg/L) were investigated in eight strains of Bacteroides fragilis. All the strains produced elevated levels of beta-lactamase. Four B. fragilis strains produced metallo-beta-lactamase capable of marked imipenem hydrolysis, and these enzymes were shown to be responsible for resistance. There was evidence, from testing sus...

background and objectives : the rapid emergence and dissemination of carbapenemase-producing klebsiella pneumoniae strains and other members of the enterobacteriaceae poses a considerable threat to the care of hospitalized patients and to public health. the aim of this study was to determine the frequency of metallo-β-lactamases (mbl) and vim-1 gene in multidrug-resistant strains of k. pneumoni...

Objective: In addition to assessing the phenotypic development of biofilms and beta-lactamases, in current study, Pseudomonas aeruginosa isolates from intensive care units (ICU) patients with prevalent drug resistance are being evaluated. Methods: Standard CLSI recommended protocols were utilized detect beta-lactamases such as extended-spectrum β-lactamases (ESBL), metallo beta-lactamase (MBL),...

Klebsiella pneumoniae strains co-producing klebsiella pneumoniae carbapenemase (KPC) and verona integron-encoded metallo-beta-lactamase (VIM) are frequently isolated in Greece and have also occurred in other European countries. Conventional combined disc tests exhibit low sensitivity against these emerging pathogens. We have evaluated modifications of the KPC/Metallo-β-Lactamase Confirmation ki...

The main objective is to study the genotypic and characterization of plasmid deoxyribonucleic acid content resistant isolates in Erbil city. A total 39 Acinetobacter baumannii were isolated from 102 diabetic foot ulcer patients, whose ages ranged 37 88 y, attending various medical wards hospitals city, Iraq, between May 2020 March 2021. Isolates tested for presence extended-spectrum beta-lactam...

Background: Acinetobacter baumannii, is an opportunistic pathogen and is responsible for numerous nosocomial infections. In recent years, this microorganism has been resistant to a wide range of antibiotics. One of the most important mechanisms of resistance in this microorganism is production of metallo-beta-lactamases (MBLs). Objectives: The aim of this study was to detect VIM- and IMP-type ...