Cytomorphometry made on cytological slides is the quantitative method of precise analysis of cellular structures, including both cytoplasm and nucleus. The aim of this study was to describe cytomorphometric parameters of mesothelial cells in the course of benign reactive and malignant proliferation and to compare them to carcinomas and adenocarcinomas located within serosal cavities in dogs. Th...

During development, the lung mesoderm generates a variety of cell lineages, including airway and vascular smooth muscle. Epigenetic changes in adult lung mesodermal lineages are thought to contribute towards diseases such as idiopathic pulmonary fibrosis and chronic obstructive pulmonary disease, although the factors that regulate early lung mesoderm development are unknown. We show in mouse th...

Most internal organs are situated in a coelomic cavity and are covered by a mesothelium. During heart development, epicardial cells (a mesothelium) move to and over the heart, undergo epithelial-mesenchymal transition (EMT), and subsequently differentiate into endothelial and vascular smooth muscle cells. This is thought to be a unique process in blood vessel formation. Still, structural and de...

AIM To investigate the prevalence and amount of polyploidy in fine needle aspiration specimens of the liver, urinary cytospin preparations, and cytospin preparations from pleural and peritoneal fluid. METHODS Cells from 44 liver smears, 48 urine specimens, and 46 pleural and peritoneal aspirations were examined. After Feulgen restaining the DNA content of 100 randomly selected nuclei was dete...

EXPERIMENTAL SUBSTANTIATION OF SELECTING THE PRODUCT FOR SANITATION OF ABDOMINAL CAVITY IN ACUTE GENERALIZED PERITONITIS V.V. Boyko, V.K. Logachev, N.A. Remneva, M.E. Tymchenko, V.P. Nevzorov, O.A. Golovina The work has studied morphological and ultrastructural changes in the mesothelium of rat peritoneum and bacterial contamination after sanitation of abdominal cavity by 0.3% solution of H2O2 ...

A panel of seven monoclonal antiepithelial antibodies of different specificities, including anticytokeratin, human milk fat globule membrane, Ca, and carcinoembryonic antigen (CEA) were used with the alkaline phosphatase-antialkaline phosphatase (APAAP) immunostaining technique to determine their value in the differentiation between benign and malignant mesothelial cells and lung carcinoma in h...

A panel of seven monoclonal antiepithelial antibodies of different specificities, including anticytokeratin, human milk fat globule membrane, C, and carcinoembryonic antigen (CEA) were used with the alkaline phosphatase-antialkaline phosphatase (APAAP) immunostaining technique to determine their value in the differentiation between benign and malignant mesothelial cells and lung carcinoma in hi...

Apparent permeability to albumin (P(alb)) was measured with (125)I-albumin in specimens of rabbit parietal pericardium from lumen to interstitium (L-I) and from interstitium to lumen (I-L). With albumin concentration (C(alb)) 0.5%, P(alb) (x 10(-5) cm/s) L-I at 37 degrees C was 0.172 +/- 0.019 SE; it decreased to 0.092 +/- 0.022 I-L at 37 degrees C, 0.089 +/- 0.021 L-I at 12 degrees C, and 0.08...

The ability of the encapsulated species Bacteroides fragilis to adhere to rat peritoneal mesothelium was compared to the adherence of unecapsulated strains of Bacteroides (B. distasonis, B. vulgatus, B. thetaiotaomicron, B. ovatus, and B. "other"). Adherence was assayed by attaching plexiglas plates containing 8-mm holes to the peritoneal mesothelium of anesthetized rats. Cell suspension (0.25 ...

Lungs develop from paired endodermal outgrowths surrounded by a mesodermal mesenchyme. Part of this mesenchyme arises from epithelial-mesenchymal transition of the mesothelium that lines the pulmonary buds. Previous studies have shown that this mesothelium-derived mesenchyme contributes to the smooth muscle of the pulmonary vessels, but its significance for lung morphogenesis and its developmen...