Abbreviations: ACN: Acetonitrile; AvBD: Avian Beta-Defensin; CAM: Carbamidomethylation; 1,5 DAN: 1,5 Diaminonaphthalene; DHB: 2,5 Dihydroxybenzoicacid; DTT: Dithiothreitol; FITC: Fluorescein Isothiocyanate; IAA: Iodoacetamide; ISD: In Source Decay; MALDI-MS: Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; m/z: Mass/Charge; PI: Propidiumiodide; RP-HPLC: Reverse Phase-High Pressure...

Matrix Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry (MS) with a computer-controlled xy-stage in the ionization source can be used to detect the presence, approximate amount and location of small molecules, lipids, peptides and proteins in tissue samples through in vitro molecular imaging, often referred to as MALDI imaging. Both MALDI Time-of-flight/Time-of-flight (MALDI-TOF/T...

Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identificatio...

The rapid identification of the potentially toxigenic Corynebacterium species, C. diphtheriae, C. ulcerans and C. pseudotuberculosis is essential for diagnosis and treatment of diphtheria and diphtheria-like diseases. We used matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDIT-OF MS) in comparison with classical microbiological and molecular methods on 116 Coryn...

A new tandem time-of-flight (TOF-TOF) instrument has been developed by modifying a standard matrix-assisted laser desorption ionization (MALDI)-TOF instrument to make high-performance, high-energy collision-induced dissociation (CID) MALDI tandem mass spectrometry (MS) a practical reality. To optimize fragment spectra quality, the selected precursor ion is decelerated before entering a floating...

AIM The performance of Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying species of nontuberculous mycobacteria (NTM) in flagged Mycobacteria Growth Indicator Tubes (MGIT)s is unclear. MATERIALS & METHODS A total of 92 sequential MGIT-positive nontuberculous mycobacteria isolates, including Mycobacterium intracellulare, ...

The performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulte...

Unbiased species-level identification of coagulase-negative staphylococci (CoNS) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified Staphylococcus lugdunensis to be a more commonly isolated CoNS in our laboratory than previously observed. It has also highlighted the possibility of vertical transmission.

We report the case of a nosocomial infection due to Enterococcus cecorum isolated from a blood culture of a 75-year-old septic male patient. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) and Vitek 2 succeeded in identification of the isolate.

We studied the possibility of detection [Ru(η5-C5H5)(PPh3)2Cl] (abbreviated by RuCp) complex as a model system for Ru-based metallodrugs in human urine using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) without previous purification or removal inorganic salts. Inorganic salts might prevent RuCp MALDI-TOF MS, most likely through increased number and...