نتایج جستجو برای: maltogenic amylase
تعداد نتایج: 10926 فیلتر نتایج به سال:
We enzymatically modified rice starch to produce highly branched amylopectin and amylose and analyzed the resulting structural changes. To prepare the highly branched amylopectin cluster (HBAPC), we first treated waxy rice starch with Thermus scotoductus alpha-glucanotransferase (TSalphaGT), followed by treatment with Bacillus stearothermophilus maltogenic amylase (BSMA). Highly branched amylos...
Amylases catalyze the hydrolysis of starch material and play central roles in carbohydrate metabolism. Compared with many different amylases that are able to hydrolyze only alpha-D-(1,4)-glycosidic bonds, maltogenic amylases exhibit catalytic versatility: hydrolysis of alpha-D-(1,4)- and alpha-D-(1,6)-glycosidic bonds and transglycosylation of oligosaccharides to C3-, C4-, or C6-hydroxyl groups...
The maltose α-amylase AmyM from Bacillus stearothermophilus can be used for flour modification, baked goods preservation, and production. Here, we optimized the recombinant expression of in subtilis WB800 via several strategies. By screening optimal promoter, a double promoter combination (P43 PamyL) could improve level by 61.25%, compared with strong P43. Then, secretion efficiency over-expres...
The physiological functions of two amylolytic enzymes, a maltogenic amylase (MAase) encoded by yvdF and a debranching enzyme (pullulanase) encoded by amyX, in the carbohydrate metabolism of Bacillus subtilis 168 were investigated using yvdF, amyX, and yvdF amyX mutant strains. An immunolocalization study revealed that YvdF was distributed on both sides of the cytoplasmic membrane and in the per...
Acarbose is a well-known inhibitor of α-glucosidases, α-amylases, cyclomaltodextrin glucanyltransferase (CGTase), and dextransucrase. Bacillus stearothermophilus maltogenic amylase (BSMA) was found to hydrolyze acarbose to give acarviosine-glucose plus glucose and to carry out a transglycosylation reaction to give isoacarbose. Acarviosine-glucose was a potent inhibitor of baker’s yeast α-glucos...
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