Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is one of themost fundamental analysis techniques for proteins, peptides, and synthetic polymers to date. Along with field desorption (FD), fast atom bombardment (FAB), laser desorption coupled with time-of-flight (LD-TOF), secondary ion mass spectrometry coupled with time-of-flight (SIMS-TOF), and electr...

A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, beta-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lu...

Matrix Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry (MS) with a computer-controlled xy-stage in the ionization source can be used to detect the presence, approximate amount and location of small molecules, lipids, peptides and proteins in tissue samples through in vitro molecular imaging, often referred to as MALDI imaging. Both MALDI Time-of-flight/Time-of-flight (MALDI-TOF/T...

Since its introduction during the last decade, MALDI mass spectrometry imaging (MSI) is now a routine technique in biology. Nevertheless, a missing link exists in MALDI MSI. Lipids, peptides/proteins, metabolites and drugs can easily be mapped using MALDI-MSI, but this technique has not yet been used to map the transcriptome, which includes microRNA, siRNA and other components. This latter fiel...