Lysozyme–chitosan composite films were developed for enhancing the antimicrobial properties of chitosan films. A 10% lysozyme solution was incorporated into 2% chitosan film-forming solution (FFS) at a ratio of 0%, 20%, 60%, and 100% (w lysozyme/w chitosan). Films were prepared by solvent evaporation. Lysozyme release from the film matrix, the antimicrobial activity of films against Escherichia...

The bacteriolytic activity of interleukin-2 and chicken egg lysozyme in the presence of various substances has been studied. Glycine and lysine do not affect the activity of interleukin-2 but increase that of lysozyme, showing a bell-shape concentration dependence peaking at 1.5 mM glycine and 18 mM lysine. Arginine and glutamate activate both interleukin-2 and lysozyme with a concentration dep...

b ackground: in this study the concentration of lysozyme in blood plasma of  microtus agrestis, clethrinomys glareolus , apodemus sylvaticus, bk rats and outbred white mice before and after infection with culture forms of trypanosoma microti, t, evotomys, t. grosi, t. lewisi and t. musculi respectively was measured. m ethods: blood samples of rodents, microtus agrestis, clethrionomys glareolus,...

protein aggregation is a problem in biotechnology. high temperature is one of the most important reasons to enhance enzyme inactivation and aggregation in industrial systems. this work focuses on the effect of tio2 and sio2 nanoparticles on refolding and reactivation of lysozyme. in the presence of tio2 and sio2 nanoparticles, after enzyme heat treatment at 98◦c for 30 min, not only aggregates ...

Lysozyme interaction with an acrylic-based hydrogel, poly(2-hydroxyethyl methacrylate) co-methacrylic acid (P(HEMA-MAA)), was investigated using a combination of quartz crystal microbalance with dissipation (QCM-D), surface plasmon resonance (SPR) and dual polarisation interferometry (DPI). This combination of techniques demonstrated that lysozyme initially absorbed into the hydrogel matrix and...

Serum lysozyme activities and semiquantitative analysis of tissue lysozyme distribution were studied in patients with primary biliary cirrhosis (PBC), chronic hepatitis (CH), miscellaneous liver diseases, and normal subjects. Serum lysozyme was significantly raised in PBC and CH. Portal venous blood has similar lysozyme activities to peripheral venous blood in a group of various liver diseases....

the interaction between nano-sio2 and lysozyme was investigated by the method of uv-visible  detection and fluorescence spectroscopic techniques. the thermal denaturation of lysozyme has been investigated in the presence and absence of nano-sio2 over the temperature range (293-373) k in different buffers and ph values, using temperature scanning spectroscopy. the presence of nano-sio2 caused th...

The complexation of lysozyme and sodium (sulfamate carboxylate) isoprene/ethylene oxide (SCIEO) at pH = 7.4 and the release of lysozyme from the complexes in the presence of NaCl were investigated. Through electrostatic and hydrophobic interactions, lysozyme and SCIEO form stable complex nanoparticles. The complexation partially disturbs the structure of lysozyme. Some of the hydrophobic residu...

Lysozyme is a protein found in egg white, tears, saliva, and other secretions. As a marketable natural alternative to preservatives, lysozyme can act as a natural antibiotic. In this study, we have isolated Bacillus licheniformis TIB320 from soil, which contains a lysozyme gene with various features. We have cloned and expressed the lysozyme in E. coli. The antimicrobial activity of the lysozym...

σ factors provide RNA polymerase with promoter specificity in bacteria. Some σ factors require activation in order to interact with RNA polymerase and transcribe target genes. The Extra-Cytoplasmic Function (ECF) σ factor, σV, is encoded by several Gram-positive bacteria and is specifically activated by lysozyme. This activation requires the proteolytic destruction of the anti-σ factor RsiV via...