Germacrene C was found by GC-MS and NMR analysis to be the most abundant sesquiterpene in the leaf oil of Lycopersicon esculentum cv. VFNT Cherry, with lesser amounts of germacrene A, guaia-6,9-diene, germacrene B, b-caryophyllene, a-humulene, and germacrene D. Soluble enzyme preparations from leaves catalyzed the divalent metal ion-dependent cyclization of [1-3H]farnesyl diphosphate to these s...

Ferredoxin-glutamate synthase was localized in leaves and cotyledons of tomato (Lycopersicon esculentum, cv Hellfrucht früshstamm) seedlings by immunocytochemical methods. The present work established that the enzyme was not only a constituent of chloroplast stroma of the mesophyll cells, but also of the cells of xylem parenchyma and epidermis.

HsfA2 is a heat stress (hs)-induced Hsf in peruvian tomato (Lycopersicon peruvianum) and the cultivated form Lycopersicon esculentum. Due to the high activator potential and the continued accumulation during repeated cycles of heat stress and recovery, HsfA2 becomes a dominant Hsf in thermotolerant cells. The formation of heterooligomeric complexes with HsfA1 leads to nuclear retention and enha...

Chimeric genes containing a pollen-specific promoter from tomato (Lycopersicon esculentum) or the CaMV35S promoter were transiently expressed following their introduction into tobacco (Nicotiana tabacum) pollen using high velocity microprojectiles. Transient expression of the microprojectile-introduced genes in leaves and pollen was similar to that observed for these genes in stably transformed...

Leakage rates were determined from leaf cells loaded with rubidium and [(3)H]leucine. There was a differential response between leucine and rubidium leakage depending upon the species used. The rate of leucine leakage shows a small decline below 5 C for two altitudinal variants of Lycopersicon hirsutum Humb. and Bonpl., whereas Lycopersicon esculentum L. showed a marked increase below 5 C. Rubi...