Cellular production of ribosomes involves the formation of highly defined interactions between ribosomal proteins (r-proteins) and ribosomal RNAs (rRNAs). Moreover in eukaryotic cells, efficient ribosome maturation requires the transient association of a large number of ribosome biogenesis factors (RBFs) with newly forming ribosomal subunits. Here, we investigated how r-protein assembly events ...

The complete gene sequence and secondary structure of the mitochondrial LSU rRNA from the cultivated Basidiomycota Agrocybe aegerita was derived by chromosome walking. The A.aegerita LSU rRNA gene (13 526 nt) represents, to date, the longest described, due to the highest number of introns (eight) and the occurrence of six long nucleotidic extensions. Seven introns belong to group I, while the i...

Previous studies using the nuclear SSU rDNA have indicated that the photosynthetic euglenoids are a monophyletic group; however, some of the genera within the photosynthetic lineage are not monophyletic. To test these results further, evolutionary relationships among the photosynthetic genera were investigated by obtaining partial LSU nuclear rDNA sequences. Taxa from each of the external clade...

INTRODUCTION This compilation is part of an ongoing effort to maintain a comprehensive and continually updated collection of large subunit (LSU; 23S and 23S-like) rRNA secondary structures and associated sequence and citation information. Table 1 gives a breakdown of the number and phylogenetic distribution of sequences currently in this LSU rRNA database. A listing of accession numbers for all...

BACKGROUND Polyporus umbellatus is an important medicinal fungus distributed throughout most area of China. Its wide distribution may have resulted in substantial intraspecific genetic diversity for the fungus, potentially creating variation in its medical value. To date, we know little about the intraspecific genetic diversity of P. umbellatus. METHODOLOGY/PRINCIPAL FINDINGS The objective of...

We previously proposed a mechanism for control of Rubisco expression and assembly during oxidative stress in Chlamydomonas reinhardtii. The N terminus of the large subunit (LSU) comprises an RNA recognition motif (RRM) that is normally buried in the protein, but becomes exposed under oxidizing conditions when the glutathione pool shifts toward its oxidized form. Thus, de novo translation and as...

We previously proposed a novel mechanism for control of ribulose-1,5 biphosphate carboxylase/oxygenase (Rubisco) expression and assembly during oxidative stress in Chlamydomonas reinhardtii. The N-terminus of the large subunit (LSU) comprises an RNA recognition motif (RRM) that is normally buried in the protein, but becomes exposed under oxidizing conditions, when the glutathione pool shifts to...

The expression of the genes encoding the large subunit (LSU) and small subunit (SSU) of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was examined in roots, stems, cotyledons, and leaves of amaranth during the development of these tissues. The highest accumulation of LSU and SSU polypeptides occurred in cotyledons and leaves. Their steady state levels were approximately 20-fold lower in stem...

Ribulose bisphosphate carboxylase/oxygenase was purified to apparent homogeneity from the carboxysomes of Prochlorothrix hollandica. The MW of the native enzyme was estimated to be 560,000 Dalton, comprising large subunits (LSU) of 57,000 Dalton and small subunits (SSU) of 13,000, probably in an 8LSU8SSU quaternary structure. Enzyme activity was maximal at pH 8.0 at 30 degrees C. The requiremen...