background: corn silk (cs) is widely used in iranian traditional medicine. the aim of this study was to investigate hepatoprotective activity of cs by isolated rat liver perfusion system (irlp). methods: hydro-alcoholic extract of corn silk (10, 20, 40, and 100 mg kg-1) was evaluated for its hepatoprotective activity by irlp. phenol and flavonoid contents of the extract were determined as galli...

A kidney perfusion machine, model MOX-100 (Waters Instruments, Ltd, Rochester, MN) was modified to allow continuous perfusion of the portal vein and pulsatile perfusion of the hepatic artery of the liver. Additional apparatus consists of a cooling system, a membrane oxygenator, a filter for foreign bodies, and bubble traps. This system not only allows hypothermic perfusion preservation of the l...

introduction: suspension of isolated hepatocyes is now being used in an increasing number of biochemical investigations, including drug metabolism and toxicity studies. isolated hepatocyes, retain many of the essential properties of the intact tissue including permeability charactreristics. material and methods: in this method at first liver is subjected to a nonrecirculating perfusion with cal...

Drug testing, either on animals or 2D cell cultures, has its limitations due to inaccurate mimicking of human pathophysiology. The liver, as one the key organs that filters and detoxifies blood, is susceptible drug-induced injuries. Integrating 3D bioprinting with microfluidic chips fabricate organ-on-chip platforms for liver cultures continuous perfusion can offer a more physiologically releva...

Two patients in deep hepatic coma due to fulminant viral hepatitis were treated by extracorporeal baboon liver perfusion after failing to respond to medical treatment and three consecutive exchange transfusions. Both patients recovered full consciousness after one liver perfusion, made a complete recovery, and were leading normal lives seven and eight months after treatment. Perfusions were mai...

The binding of hepatic lipase to rat liver was studied in an ex vivo perfusion model. The livers were perfused with media containing partially purified rat hepatic lipase or bovine milk lipoprotein lipase. The activity of the enzymes was determined in the perfusion media before and after passage through the liver. During perfusion with a hepatic-lipase-containing medium the lipase activity in t...

BACKGROUND Liver microcirculation can be affected by a wide variety of causes relevant to liver transplantation and resectional surgery. Intraoperative assessment of the microcirculation could possibly predict postoperative outcome. The present pilot study introduces laser speckle contrast imaging (LSCI) as a new clinical method for assessing liver microcirculation. METHODS LSCI measurements ...

One strategy for assessing efficacy of a liver transplant is to monitor perfusion and oxygenation after transplantation. An implantable optical sensor is being developed to overcome inadequacies of current monitoring approaches. To facilitate sensor design while minimizing animal use, a polydimethylsiloxane (PDMS)-based liver phantom was developed to mimic the optical properties of porcine live...

BACKGROUNDS/AIMS Conventional graft perfusion method using one small-caliber catheter takes a relatively long time for right liver graft perfusion, thus some modification is needed. In this study, we intended to assess the effectiveness of right liver graft perfusion methods through comparison of different infusion catheters. METHODS The study consisted of two parts including one bench experi...

Donation after circulatory death (DCD) grafts are commonly used in liver transplantation. Attributable to the additional ischemic event during donor warm ischemia time (DWIT), DCD carry an increased risk for severe ischemia/reperfusion injury and postoperative complications, such as cholangiopathy. The actual DWIT depends on course of vital parameters withdrawal life support varies widely betwe...