The potential use of liposomes as a delivery system is still limited by the poor understanding of their interaction mechanisms with biological media. In the present work, interaction between bovine albumin (BA) and liposomes was studied using phase transition and dielectric measurements as well as solubilization process using non-ionic detergent octylglucoside (OG). After liposomes were incubat...

The carrier facilitated transport of lithium picrate was studied using a series of non-cyclic polyethers containing different end groups and chain lengths through Bulk Liquid Membrane (BLM) and Supported Liquid Membrane (SLM) systems. The various membrane supports used are viz. PTFE, cellulose nitrate, and dialysis membrane and onion membrane. The amount of Li+ transported depends upon the stru...

The effect of Triton X-100, Na cholate and Tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of Salmonella typhimurium was studied. The detergents were used in various concentrations (1.6 to 64 mM) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. Results showed that no inne...

Studying membrane proteins represents a major challenge in protein biochemistry, with one of the major difficulties being the problems encountered when working outside the natural lipid environment. In vitro studies such as crystallization are reliant on the successful solubilization or reconstitution of membrane proteins, which generally involves the careful selection of solubilizing detergent...

background and objectives: phosphorus is one of the low bioavailable macroelements. use of microorganisms in biofertilizers could release phosphorus from insoluble compounds. pseudomonas putida p13 and pantoea agglomerans p5 are well recognized for application as phosphate solubilizing bioinoculants and are used as solid carrier based. liquid bioinoculants are preferred for economizing producti...

We have observed large pores in the membrane of giant vesicles in an aqueous medium. The lifetime of the pores can reach 2 min and their size (a few micrometers) enables their visualization by fluorescence microscopy. These pores are obtained thanks to a destabilization of the membrane due to the synergistic action of a cone-shaped and nitrobenzodiazole (NBD) labeled phospholipid illuminated in...

Porous surface modified polyvinylidene ï‌‚uoride (PVDF) hollow fiber membranes are fabricated through a dry-wet phased inversion process. Surface modifying macromolecules (SMM) (1 wt. %) are used as additives in the spinning dope. The performance of the surface modified membrane in contactor application for CO2 stripping from water is assessed through the fabricated gas–liquid membrane contacto...

We establish a biophysical model for the dynamics of lipid vesicles exposed to surfactants. The solubilization of the lipid membrane due to the insertion of surfactant molecules induces a reduction of membrane surface area at almost constant vesicle volume. This results in a rate-dependent increase of membrane tension and leads to the opening of a micron-sized pore. We show that solubilization ...

Integral membrane proteins (IMPs) play crucial roles in all cells and represent attractive pharmacological targets. However, functional and structural studies of IMPs are hindered by their hydrophobic nature and the fact that they are generally unstable following extraction from their native membrane environment using detergents. Here we devise a general strategy for in vivo solubilization of I...