The synthesis of biodiesel from sunflower oil and methanol based on transesterification using the immobilized lipase from Thermomyces lanuginosus (Lipozyme TL-IM) has been investigated under silent conditions and under an ultrasound field. Ultrasound assisted process led to reduced processing time and requirement of lower enzyme dosage. We found for the first time that oil to methanol ratio of ...

The enzymatic transesterification of several triand tetrasaccharides with vinyl laurate is described. The lipases from Candida antarctica B (Novozym 435) and Thermomyces lanuginosus (Lipozyme TL IM) and the alkaline protease from Bacillus licheniformis (subtilisin Carlsberg) have been used with each carbohydrate to obtain different regioisomers. By using the sugars in their amorphous form, comp...

c-Decalactone is an industrially interesting peach-like aroma compound that can be produced biotechnologically through the biotransformation of ricinoleic acid. Castor oil (CO) is the raw material most used as the ricinoleic acid source. The effect of different CO concentrations on the c-decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L was found to ...

Structured lipids (SL) were synthesized by the acidolysis of borage oil with caprylic acid using lipases. Six commercial lipases from different sources and a novel lipase from Pichia lynferdii NRRL Y-7723 were screened for their acidolysis activities and Lipozyme RM IM and NRRL Y-7723 lipase were selected to synthesize symmetrical SL since recently NRRL Y-7723 lipase was identified as a novel c...

N-methyl fatty hydroxamic acids (N-MFHAs) is a derivative of acid (FHA), were synthesized from ketapang seed oil (Terminalia catappa L.) with hydroxylamine enzymatically using immobilized lipase (Lipozyme TL IM). N-MFHAs that are still in mixed state triglycerides the oil. This research aims to determine optimum conditions for separation into their single components and percentage composition H...

A new micro-procedure for the quick regiospecific analysis of triacylglycerols (TAG) with a 1,3-specific lipase, Lipozyme IM 20 (produced from strains of Muccor miehei) is described. After dissolution of triacylglycerols in butan-1-ol or butan-2-ol 250 mg of Lipozyme IM 20 were added and the whole mixture was agitated. This operation was repeated 5-10 times and the esterified fatty acids in the...

* corresponding author. e-mail: [email protected] Phenethyl octanoate (PEO) is a natural flavour compound found in rum, spirits and wine. Six commercial and non-commercial lipase preparations were screened for 2-phenethyl octanoate synthesis activity, the most active enzymes were Lipozyme® RM IM and Palatase® 20000 L (immobilized and soluble Rhizomucor miehei lipases). Three substrates a...