Lipoprotein lipase activity in adipose tissue responds rapidly to changes in the physiological state. To study what mechanisms are involved in the regulation, guinea pigs were fasted and the decrease in adipose-tissue lipoprotein lipase activity was compared with the decreases in mRNA and lipase synthesis. The mRNA pattern (three species) did not change. There was a close parallelism between th...

cloning and sequencing of a lipase gene from an indigenous bacillus pumilus, strain f3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. sequence analysis showed that f3 lipase contained a signal peptide composed of 34 amino acids with an h domain of 18 residues. a tat-like motif was found in the signal peptide similar to some other bacillus pumil...

The -514 polymorphism in the hepatic lipase gene (LIPC) is associated with decreased hepatic lipase activity. In the present study, the interaction between body mass index (BMI), the -514 polymorphism, and hepatic lipase activity was examined in 118 white men and in 51 African American men. BMI was significantly positively correlated with hepatic lipase activity in both populations. BMI was sim...

The use of proteins and peptides as human therapeutics has been increased in recent years. Lipase is a relatively homogeneous proteinaceous enzyme indicated in maldigestion. The purpose of the present study was to evaluate the effect of humidity along with compactional pressure on the enzymatic activity of wheat germ lipase. Samples of lipase powder were kept at different relative humidity (RH)...

The ontogenesis and specific activities of pancreatic (trypsin, chymotrypsin, amylase and lipase) and intestinal enzymes (alkaline phosphatase, aminopeptidase N) were investigated in Kutum (Rutilus frisii Kutum) from the onset of exogenous feeding (3 day after hatching, DAH) to the juvenile stage at 50 DAH. Trypsin- and chymotrypsin-specific activity showed similar patterns and increased with l...

The presence of NaCl-resistant, neutral triacylglycerol hydrolase (lipase) activity in rat adrenal gland, ovary and testis was studied. Both adrenals and ovaries but not testes were found to contain such a lipase. The activity of the enzyme in the adrenal gland was lowered during cortisol treatment and hypothyroidism. An elevated adrenal lipase activity was found during hyperthyroidism. Pseudo-...

1. A selective immunochemical method was used to measure post-heparin plasma lipoprotein lipase and hepatic lipase activity in eighty-two normal subjects and in twenty patients with type IIb, IV or V hypertriglyceridaemia. In twenty-six normal subjects the activity of post-heparin plasma lipases was compared with the kinetic parameters of endogenous plasma triglyceride metabolism. 2. The activi...

Addition of heparin to isolated fat cells incubated at 23” resulted in a 3-fold increase in release of lipoprotein lipase activity into the incubation medium. This increase in medium lipoprotein lipase activity was not due to enhancement or stabilization of lipase activity present in the medium. The maximum increase in lipoprotein lipase activity was obtained on addition of 0.1 unit of heparin ...

Addition of heparin to isolated fat cells incubated at 23” resulted in a 3-fold increase in release of lipoprotein lipase activity into the incubation medium. This increase in medium lipoprotein lipase activity was not due to enhancement or stabilization of lipase activity present in the medium. The maximum increase in lipoprotein lipase activity was obtained on addition of 0.1 unit of heparin ...