background : there are several methods, such as vaccination, to control visceral leishmaniasis. although there is no efficient vaccine, it seem dna vaccination with stimulates both cellular and humoral immunity apparently is the best way. the aim of this study was cloning and expression of lack gene, a 36kd protein, as a can-didate protein for vaccination against iranian l.infantum. methods: ir...

clostridium perfringens is a gram-positive, obligate anaerobic bacterium, which is widely distributed in the environment. c. perfringens is subdivided to 5 groups (types a to e), based on its four major toxin (alpha, beta, epsilon and iota). c. perfringens type b beta toxin causes inflammation and bloody necrotic enteritis. type b related enterotoxaemia is a major problem of veterinary sciences...

colonization of nontypeable haemophilus influenzae (nthi) in nasopharynx causes respiratory tract disease. in 80% of clinical isolates, hmw proteins are the major adhesions and induce protective antibodies in the hosts. therefore, it can be used as a vaccine candidate. the aim of this study is designing and cloning of the conserved regions of nthi hmw1 core binding domain.in this study, the sta...

objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

background : echinococcosis or hydatidosis is a chronic, zoonotic worldwide infection caused by the larval stage of the dog taeniid tapeworm echinococcus granulosus. vaccination has been considered as one of the ways to prevent of hy-datidosis in recent decades. the aim of this study was to construct a pcdna3.1 eukaryotic expression vector contain-ing the subunit 8-kda antigen b (hyd1) of e. gr...