A 60-year-old Muslim female was admitted to hospital on January 1, 1965, with the complaint of a gradually increasing mass over the cornea of the left eye for the last 8 years. There was no history of venereal disease, chronic septic foci, or tuberculosis. Examination.-Her general health was normal; the liver and spleen were not enlarged. The lids of the left eye were normal. A growth covered t...

15 years old male reported with diminution of vision in the left eye of three months duration. He had a cystic lesion in the inferonasal area of the cornea straddling the limbus, partially on the cornea and partially on the sclera. Surgical excision of the lesion revealed it to be a continuous intrascleral and intracorneal cyst. Histopathology confirmed it to be a calcified cysticercosis.

Corneal neovascularization (CoNV), a pathological form of angiogenesis, involves the growth blood and lymph vessels into avascular cornea from limbus adversely affects transparency vision. Alkali burn is one most common forms ocular trauma that leads to CoNV. In this protocol, CoNV experimentally induced using sodium hydroxide solution in controlled manner ensure reproducibility. The alkali mod...

PURPOSE When treating peripheral ectatic disease-like pellucid marginal degeneration (PMD), corneal cross-linking with UV-A and riboflavin (CXL) must be applied eccentrically to the periphery of the lower cornea, partly irradiating the corneal limbus. Here, we investigated the effect of standard and double-standard fluence corneal cross-linking with riboflavin and UV-A (CXL) on cornea and corne...

PURPOSE To elucidate the unique molecular factors and biological processes that are differentially expressed in the limbal stem cell microenvironment by comparing directly to that of its immediate adjacent structures, the cornea and conjunctiva. METHODS Total RNA was isolated and amplified from the limbus, cornea, and conjunctiva. A gene expression profile of each tissue type was obtained by ...

PURPOSE To study the expression of intermediate filaments in the human cornea. METHODS Light and electron microscopic and immunohistochemical studies were performed on 20 corneas from subjects of various ages. RESULTS A hitherto unrecognized epithelial cell population emerged from the immunohistochemical studies. Epithelial cells were invariably present in the superior cornea, whereas the n...

The objective of this study was to reproducibly measure corneal epithelial thickness centrally and at the limbus in the rabbit cornea using ultrahigh resolution optical coherence tomography (OCT). Twelve freshly enucleated New Zealand white rabbit eyes were kept in a moist chamber at 4 degrees C. An ultrahigh resolution OCT system with a spatial resolution of 1.3 microm was used to image the co...

The authors studied the distribution of specific keratins within the superior, inferior, medial, and lateral regions of human limbus and cornea to determine whether the limbal epithelium exhibits regional heterogeneity in its microstructure. A corneal epithelial basic keratin (K3), recognized by monoclonal antibody AE5, was immunohistochemically undetectable in the basal layers of the limbus in...

Corneal epithelial stem cells or limbal stem cells (LSCs) are responsible for the maintenance of the corneal epithelium in humans. The exact location of LSCs is still under debate, but the increasing need for identifying the biological processes in the limbus, where LSCs are located, is of great importance in the regulation of LSCs. In our current study we identified 146 preferentially expresse...

The limbus is the structurally rich transitional region of tissue between the cornea on one side, and the sclera and conjunctiva on the other. This zone, among other things, contains nerves passing to the cornea, blood and lymph vasculature for oxygen and nutrient delivery and for waste, CO(2) removal and drainage of the aqueous humour. In addition, the limbus contains stem cells responsible fo...