Enzyme mimetic is an increasing pull to overcome the limitations on natural enzymes. mimicking done with help of nanozymes. Nanozymes contain nonmaterial which have enzyme-like activity; this similar activity attracts researchers.

In this work, an efficient synthesis of bionanohybrids as artificial metalloenzymes (Cu, Pd, Ag, Mn) based on the application enzyme a scaffold was described. Here we evaluated effect changing metal, pH medium, and amount in these metalloenzymes, where changes metal species size nanoparticles occur. These nanozymes were applied degradation hydrogen peroxide for their evaluation mimetics catalas...

We report the development and initial experimental validation of a computational design procedure aimed at generating enzyme-like protein catalysts called "protozymes." Our design approach utilizes a "compute and build" strategy that is based on the physical/chemical principles governing protein stability and catalytic mechanism. By using the catalytically inert 108-residue Escherichia coli thi...

Early in this study, an α-Amylase from Bacillus megaterium WHO (BMW) was isolated from hot springs of Ramsar (North of Iran), and its gene was cloned in E.coli. Based on its conserved sequence regions and substrate specificity, it was classified as intermediary group enzymes with the specificity of oligo-1,6-glucosidase and neopullulanase subfamilies. In the sixth conserved re...