In vitro cultivation of Leishmania parasites plays an important role in diagnosis and treatment leishmaniasis vaccine drug development studies. There is no information about the effects urine within culture on infectivity parasites. In this study we used two media with different overlays to enhance growth rates donovani.

BACKGROUND Visceral leishmaniasis (VL), a widely distributed systemic disease caused by infection with the Leishmania donovani complex (L. donovani and L. infantum), is almost always fatal if symptomatic and untreated. A rapid point-of-care diagnostic test for anti-Leishmania antibodies, the rK39-immunochromatographic test (rK39-ICT), has high sensitivity and specificity in South Asia but is le...

Histone post-translational modifications (PTMs) such as acetylation and methylation are known to affect chromatin higher order structures. Primary targets of these modifications include basic residues present at N-terminus tail region of core histones. Four histone acetyltransferase (HAT) genes have been identified in trypanosomatids. HAT1, HAT3 and HAT4 of Leishmania donovani have been partial...

Plasmodium falciparum Amebae (Intestinal) Babesia species Entamoeba histolytica Flagellates (Leishmaniae, Trypanosomes) Entamoeba dispar* Leishmania tropica complex Entamoeba hartmanni Leishmania mexicana complex Entamoeba coli Leishmania braziliensis complex Entamoeba polecki Leishmania donovani complex Endolimax nana Leishmania peruviana Iodamoeba bütschlii Trypanosoma brucei gambiense Blasto...

We have developed a PCR assay that in a single reaction distinguishes between Leishmania infantum and Leishmania donovani strains on the basis of different size of the amplicon. The targeted intergenic region between putative biopterin transporter and nucleotide binding protein on chromosome 35 is highly variable, species-specific and can be amplified from clinical samples. Based on the assay, ...

UNLABELLED The prokaryotic CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9, an RNA-guided endonuclease, has been shown to mediate efficient genome editing in a wide variety of organisms. In the present study, the CRISPR-Cas9 system has been adapted to Leishmania donovani, a protozoan parasite that causes fatal human visceral leishmaniasis. We introduced the Cas9 nuclease ...

We used the cysteine proteinase B (cpb) gene family of the trypanosomatid genus Leishmania as a target to develop rapid, specific, and easy-to-use polymerase chain reaction (PCR) tests to discriminate Leishmania infantum, Leishmania donovani, Leishmania tropica, Leishmania aethiopica, and Leishmania major. Identification of all 5 Old World species and validation of intraspecies variability are ...

Host-pathogen interaction is an area of considerable interest. Intracellular parasites such as Leishmania reside inside phagocytes such as macrophages, dendritic cells and neutrophils. Macrophages can be activated by cytokines such as IFN-γ and Toll like receptor (TLR) agonists resulting in enhanced microbicidal activity. Leishmania parasites hijack the microbicidal function of macrophages, mai...

The global distribution of leishmaniasis is rapidly expanding into new geographic regions. Dogs are the primary reservoir hosts for human visceral leishmaniasis caused by infection with Leishmania infantum. Natural infections with other Leishmania spp. can occur in dogs, but their role as reservoir hosts for other species of Leishmania is uncertain. Leishmania donovani is traditionally consider...

Visceral leishmaniasis (VL), is an infection due to obligate intracellular protozoa of the genus Leishmania[1]. Natural transmission of the parasite happens primarilyby way ofthe bite of contaminated female of phlebotome. In the Old World it is represented by the genus Phlebotomous but in the New World it is called Lutzomyia. Another way of transmission may occur among the intravenous drug abus...