Tea leaf color is not only important from an aesthetics standpoint but is also related to tea quality. To investigate the molecular mechanisms that determine tea leaf color, we examined Camellia sinensis cv. 'Anjin Baicha' (an albino tea cultivar) by tandem mass tag isobaric labeling to generate a high-resolution proteome and acetyl-proteome atlas of three leaf developmental stages. We identifi...

chilling is an important environmental stress that influences the yield and quality of many agricultural crops. different plants use different systems to endure this stress and minimize its effects. one of these systems is enzymatic reaction. to find out more about responses of different grapevine species and cultivars to the low temperature conditions, their enzymatic changes were evaluated in...

The field of proteomics suffers from the immense complexity of even small proteomes and the enormous dynamic range of protein concentrations within a given sample. Most protein samples contain a few major proteins, which hamper in-depth proteomic analysis. In the human field, combinatorial hexapeptide ligand libraries (CPLL; such as ProteoMiner) have been used for reduction of the dynamic range...

To gain a better understanding of the biochemical basis for partitioning of photosynthetically fixed carbon between leaf and grain, a (14)CO(2) labeling study was conducted with field-grown maize plants 4 weeks after flowering. The carbon flow was monitored by separation and identification of (14)C assimilates and (14)C storage components within each tissue during the chase period (from 4 to 96...

At the eight-leaf stage, maize is highly sensitive to stresses such as drought, heat, and their combination, which greatly affect its yield. At present, few studies have analyzed maize response to combined drought and heat stress at the eight-leaf stage. In this study, we measured certain physical parameters of maize at the eight-leaf stage when it was exposed to drought, heat, and their combin...

Drought is one of the most important factors limiting crop yields around the world. Drought stress in plants, the change (increase or decrease) in the production of plant proteins. This research was carried out using bread wheat genotypes. For evaluation of leaf protein pattern in wheat, 10 genotypes were assayed with 3 replications under irrigated (non-stress) and rain-fed (stress) conditions....

Black rice (Oryza sativa L.) is considered to be a healthy food due to its high content of anthocyanins in the pericarp. The synthetic pathway of anthocyanins in black rice grains has been identified, however, the proteomic profile of leaves during grain development is still unclear. Here, isobaric Tags Relative and Absolute Quantification (iTRAQ) MS/MS was carried out to identify statistically...

Very little is known about sugarcane gene sequences. In this study, the aim is to identify sugarcane genes expressed in the leaf roll and the mature stem. Random DNA sequencing of cDNA clones allows the identification of genes and the development of Expressed Sequence Tags (ESTs). A total of 250 clones randomly selected from a leaf roll cDNA library and 112 from a mature stem library have been ...

Previous work (1) has shown that about 50 per cent of the total protein nitrogen contained in a tobacco leaf which had recently completed growth by expansion is associated with particulate structures of leaf protoplasm such as nuclei, chloroplasts, etc. The other 50 per cent of the protein nitrogen remains soluble in the presence of neutral buffers. In the analytical ultracentrifuge, the solubl...