Growth and media Liquid cultures were grown at 37°C with aeration in minimal A medium (Miller, 1992) with 0.2% glycerol or glucose as the carbon source or in LB Miller medium (Difco). Colonies were grown at 37°C on 1.5% agar plates made from LB Miller medium or minimal A medium with glycerol as the carbon source. Plasmids were maintained by supplementing media with 50 μg/mL of ampicillin, 15 μg...

vi1An Introduction1.1DNA Replication11.1.2 Leading and Lagging Strands41.1.3 Interference61.2Secondary Structures91.2.2 Quasi-palindromes101.2.3 Quasi-palindrome Mutational Reporters12References17 2Materials and Methods2.1Growth Medium212.2Strain Construction212.3Mutational Rate Deter...

Microgravity has advantages for the cultivation of tissues with high fidelity; however, tissue formation requirescellular recognition and adhesion. We tested the hypothesis that simulated microgravity does not affect celladhesion. Human colorectal carcinoma cells were cultured in the NASA Rotating Wall Vessel @WV) under lowshear stress with randomization of the gravity vector th...

ix Czech Technical University in PragueKlokner InstituteAbstract Analysis of Composite Materials with Random Microstructureby Jan Zeman In this thesis, a comprehensive approach to the modelling of composite materials witha microstructure exhibiting various types of disorder is proposed. In particular, a fibrousgraphite-epoxy composites with fibers randomly distributed wi...

Behavior tables are a visual formalism for representing synchronous systems of com-municating processes. Although behavior tables arose from hardware modeling meth-ods, they operate on arbitrarily abstract data-types. Originally conceived as an aidfor imposing architecture on behaviorally oriented specifications, behavior tables in-herited a structural algebra from the Digital D...

Self similar solutions u(x,t) = f(x//t) of the one-dimensional porousmedium equation are studied in this paper. These solutions emerge frominitial values that consist of two constant states: one non-positive forx < 0 and one non-negative for x > 0. With a diffusivity of the form|u| , we consider for m > 0 sign change solutions and for m e (-1,0]non-negative solutions. Th...

The central task in practical problem solving is to identify and choose among alterative courses of action. Computer sciencehas failed to provide adequate tools for supporting rational, effective and fair decision-making under the conditions which usuallyprevail. Especially, computer science has yet to develop models of rational decision-making in groups which adequately take intoco...

Media The motility buffer (MB) contained (/l of distilled water) K2HPO4, 11.2 g; KH2PO4, 4.8 g; (NH4)2SO4, 2 g; MgS04. 7H20, 0.25 g; PVP, 1 g; and EDTA, 0.029 g.1 LuriaBertani (LB) broth contained LB (/l of distilled water) 25 g. Tryptone medium contained (/l of distilled water) tryptone, 10 g and Nacl, 5 g. Different concentrations of chemotaxis medium was prepared by adding different amounts ...

fConttnua on ravaraa alda It nacaaaary and Idantity by block numbar)The problem of "assuring interactive consistency" is defined in [PSL].20 It is assumed that there are n isolated processors, of which at most m arefaulty. The processors can communicate by means of two-party messages, usinga medium which is reliable and of negligible delay. The sender of a messageis always i...