Asian Seabass, Lates calcarifer precocious sex change is problematic for efficient hatchery operation. Seabass grown in freshwater farms were transfer to brackish water (32 g/L) at 28 °C and fed to satiety until acclimated. Blood, brain and gonad were collected and analysed. Brain aromatase activity appeared to respond to age/size rather than environmental conditions while gonadal aromatase was...

We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive a chromosome-level assembly with a contig N50 size over 1 Mb and scaffold N50 size over 25 Mb that span ~90% of the genome. The p...

A total of 270 fingerlings of Asian sea bass (Lates calcarifer) were allotted into three equal treatments with three equal replicates for each to investigate the effect of dietary supplementation of two levels of Black cumin seeds (Nigella sativa) and Turmeric (Curcuma longa) mixture (BTM) on growth performance and blood chemistry throughout the experimental period (98 days). Fish of the first ...

Light is a key environmental factor that strongly related to fish activity and behavior. Exposure certain wavelengths known affect growth performance survival of teleost larvae juveniles. Our study aimed determine the effects exposure three light sources [red (710 nm), blue (453 nm) green (510 nm)] on performance, behavioral response, visibility (time eat, in minutes), structure retinal layer j...

Lethal and sublethal effects of ammonia toxicity to juvenile barramundi (Lates calcarifer) were investigated under laboratory conditions following the OECD guidelines for testing of chemicals. Acute toxicity was tested in a static nonrenewal system at pH 9.0 and temperature around 29 degrees C. The 24-, 48-, and 96-h LC(50) values for barramundi were 3.89, 3.67, and 3.31 mg total ammonia N L(-1...

Locating appropriate settlement habitat is a crucial step in the life cycle of most benthic marine animals. In marine fish, this step involves the use of multiple senses, including audition, olfaction and vision. To date, most investigations of larval fish audition focus on the hearing thresholds to various frequencies of sounds without testing an ecological response to such sounds. Identifying...

BACKGROUND β2-Microglobulin (β(2)M) is the light chain of major histocompatibility class I (MHC I) that binds non-covalently with the α heavy chain. Both proteins attach to the antigen peptide, presenting a complex to the T cell to be destroyed via the immune mechanism. METHODOLOGY/PRINCIPAL FINDINGS In this study, a cDNA sequence encoding β(2)M in the Asian seabass (Lates calcarifer) was ide...

This study was undertaken to define the effects of temperature on the energy and protein partial utilisation efficiencies of juvenile Barramundi. The experiment used a factorial design with four temperatures (25oC, 29oC, 32oC, and 36oC) and three ration levels (low, moderate, satiety) to examine the response of Barramundi to varying digestible energy (DE) and digestible protein (DP) intake. Ene...