Rice stripe virus (RSV) is the type member of the genus Tenuivirus, which relies on the small brown planthopper (Laodelphax striatellus Fallén) for its transmission in a persistent, circulative-propagative manner. To be transmitted, virus must cross the midgut and salivary glands epithelial barriers in a transcytosis mechanism where vector receptors interact with virions, and as propagative vir...

Cytochrome P450 monooxygenases (CYPs) usually overexpressed in resistant strain were found involved in oxidative detoxification of insecticides. In this study, an investigation was conducted to confirm if resistance irrelevant CYPs which were not overexpressed in resistant strain before, were capable of degrading insecticides. Three resistance irrelevant CYPs viz. CYP417A2v2, CYP425A1v2, and CY...

Wolbachia species are intracellular symbionts that cause reproductive alterations in arthropods. Transinfection experiments have been performed in many arthropod species to elucidate the interaction betweenWolbachia and a new host. To ease transinfection of this bacterium to new arthropodhosts,we introduced two techniques: nymphal injection insteadof embryonic injection and the use of a culture...

Persistent plant viruses usually depend on insects for their transmission; they cannot be transmitted between plants or through mechanical inoculation. However, the mechanism by which persistent viruses become pathogenic in insect vectors remains unknown. In this study, we used Rice stripe virus (RSV), its insect vector Laodelphax striatellus and host plant (Oryza sativa) to explore how persist...

The flow cytometry method was used to estimate the genome sizes of nine agriculturally important insects, including two coleopterans, five Hemipterans, and two hymenopterans. Among which, the coleopteran Lissorhoptrus oryzophilus (Kuschel) had the largest genome of 981 Mb. The average genome size was 504 Mb, suggesting that insects have a moderate-size genome. Compared with the insects in other...

The spatial distribution of crop and non-crop habitats over segmented agricultural landscapes could be used as a means to reduce insect pest populations. Seven land cover categories such as wheat, rapeseed, vegetable, water, built-up, paved road, and unsurfaced road were extracted from GeoEye satellite images dating from late May to late June of 2010. Three diversity metrics and three evenness ...

Effects of wood vinegar on the activity of various insecticides were determined by measuring the mortality of two species of rice planthoppers, Nilaparvata lugens and Laodelphax striatellus. Wood vinegar itself did not show insecticidal activity on planthoppers. When the planthoppers were treated with wood vinegar mixed with one of insecticides such as BPMC, dinotefuran, imidacloprid, carbosulf...

Rice stripe disease caused by virus (RSV) is one of the most devastating plant viruses rice and causes enormous losses in production. RSV transmitted from to small brown planthopper ( Laodelphax striatellus) a circulative–propagative manner. The recent reemergence this pathogen East Asia since 2000 has made studied over past two decades. Extensive studies have resulted substantial advances rega...