Synexin (annexin VII) is a member of the annexin family of calcium and phospholipid binding proteins that promote calcium-dependent aggregation and fusion of lipid vesicles or secretory granules. We have previously suggested that synexin may be involved in membrane fusion processes during exocytosis of lung surfactant since it promotes fusion in vitro of lamellar bodies with plasma membranes. I...

We report disorder to lamellar transition in a system of spherically symmetric particles where the interparticle potential consists of a short-ranged attraction and a longer-ranged repulsion. The system provides a simplified model for aqueous dispersions of colloidal particles and globular proteins that may exhibit stable/metastable clusters or microscopic phases. By using a non-mean-field dens...

The interactions between mineral and collagen phases in the ultrastructural level play an important role in determining the mechanical properties of bone tissue. Three types of mineral-collagen interaction (i.e., ionic interactions, hydrogen/van der Waals bonds, and van der Waals/viscous shear in opening/sliding mode, respectively) have been simulated in this study, using cohesive zone-modeling...

As hydration proceeded, the mosaic spread, or degree of misorientation, increased. This was apparent by the increasing lengths of the arcs emanating out from the h=1 and h=2 lamellar orders, shown in Fig. S1. Mosaic spread was determined by examining lamellar orders in the LAXS CCD position. Matlab was used to plot the intensity of a lamellar order as a function of η, the angle off the meridian...

The phase transition kinetics and mechanism of formation of a lamellar-crystalline phase of dipalmitoylphosphatidylethanolamine (DPPE) dispersed in different concentrations of aqueous dimethyl sulfoxide (DMSO) during cooling have been examined by differential scanning calorimetry and synchrotron X-ray diffraction techniques. In dispersions containing mole fractions of DMSO (x<0.22), the phase t...

This review article attempts to present an overview of the occurrence and function of lipid storage and secretory organelles: the lamellar bodies. Morphologically these organelles vary considerably in size (100 nm to 2400 nm); they are surrounded by a membrane and contain multilamellar lipid membranes. Lamellar bodies may also contain apolipoproteins and lytic enzymes and have an acidic pH, whi...

The inter-lamellar connectivity of the annulus fibrosus in the intervertebral disc has been shown to affect the prediction of the overall disc behaviour in computational models. Using a combined experimental and computational approach, the inter-lamellar mechanical behaviour of the disc annulus was investigated under conditions of radial loading. Twenty-seven specimens of anterior annulus fibro...

Polysynthetically-twinned TiAl crystals were deformed by compression with loading axis parallel and perpendicular to the lamellar interfaces. The deformation structures on the free surfaces were scanned using a dimension AFM with scan directions parallel and perpendicular to the lamellar interfaces. Abnormal deformation behaviors were observed to occur in both orientations. When the compression...

Monoclonal antibodies against the limiting membrane of alveolar type II cell lamellar bodies were obtained after immunization of mice with a membrane fraction prepared from lamellar bodies isolated from rat lungs. The specificity of the antibodies was investigated with Western blot analysis, indirect immunofluorescence, and electron-microscopic immunogold studies of freshly isolated or cultured...