OBJECTIVE To observe the biochemical characters and antibiotic susceptibility of isolated Staphylococcus aureus (S. auerus) strains against some conventional and traditional antibiotics. METHODS Thirty post operative pathogenic isolated S. aureus strains were used in this study. Bacterial culture was done in Mueller-Hinton broth at 37 °C. Characters of these strains were determined by traditi...

The only carbohydrate in milk is lactose, a disaccharide, which is formed of a molecule of glucose and a molecule of galactose. Cow's milk contains about 5 % lactose and human milk about 6'5-7 %. Lactose is hydrolysed to its monosaccharides principally by a specific enzyme, lactase, which is a f-galactosidase. There are also two other enzymes with ,3-galactosidase activitv, lysosomal acid 3-gal...

Enzymatic hydrolysis of lactose by -galactosidase (Lactozym) has been studied in a jacketed stirred-tank reactor (JSTR) and in a recirculation hollow-%bre bioreactor (RHFB). The hollow-%bre module was manufactured with modi%ed cellulose-acetate membrane (Cuprophan). The variables tested were: temperature (25–40◦C), lactose concentration (0.0278–0:208 M) and enzyme concentration (0.100–4:51 g l−...

SUMMARY Sodium azide, 2,4-dinitrophenol and iodoacetate did not inhibit hydrolysis of lactose by cell-free preparations of Sacchuromyces fragilis ,8-galactosidase, but with intact organisms fermentation and hydrolysis were inhibited to a similar extent. This suggests that these inhibitors may interfere with the transport of lactose into the cell. Galactose fermentation was inhibited by sodium a...

1. The ability of malnourished children to absorb lactose, sucrose and glucose was tested by perfusing the jejunum. Intestinal motility was measured simultaneously in a perfused segment by a dye dilution technique. These tests were repeated on the same children after 6-16 weeks of treatment. 2. There was a significant correlation between the rate of hydrolysis of disaccharide perfusing the jeju...

Fermentation of lactose in whey permeate directly into ethanol has had only limited commercial success, as the yields and alcohol tolerances of the organisms capable of directly fermenting lactose are low. This study proposes an alternative strategy: treat the permeate with acid to liberate monomeric sugars that are readily fermented into ethanol. We identified optimum hydrolysis conditions tha...

Cells of Lactobacillus bulgaricus, Escherichia coli, and Kluyveromyces (Saccharomyces) lactis immobilized in polyacrylamide gel beads retained 27 to 61% of the beta-galactosidase activity of intact cells. Optimum temperature and pH and thermostability of these microbial beta-galactosidases were negligibly affected by the immobilization. Km values of beta-galactosidase in immobilized cells of L....