To improve production of fuel ethanol from renewable raw materials, laccase from the white rot fungus Trametes versicolor was expressed under control of the PGK1 promoter in Saccharomyces cerevisiae to increase its resistance to phenolic inhibitors in lignocellulose hydrolysates. It was found that the laccase activity could be enhanced twofold by simultaneous overexpression of the homologous t-...

Laccase belongs to the superfamily of multicopper oxidases and has been widely investigated in recent decades. Due its mild efficient oxidation substrates, laccase successfully applied organic catalytic synthesis, degradation harmful substances, other green fields. Nevertheless, there are few reports on catalysis with laccase. This review focuses reporting collating some latest interesting lacc...

Various basidiomycetes and deuteromycetes, grown in liquid and solid culture media, were compared for their laccase-producing ability and for the inducing effect of soil and agricultural residues on laccase production. Laccase activity in extracts of all solid media was higher than that of the liquid media. In liquid cultures, fungal laccase activity in extracts of pea (Pisum sativum) straw-tre...

A 59 kDa laccase with inhibitory activity against HIV-1 reverse transcriptase (IC(50) = 2.4 μM) was isolated from the broth of mycelial culture of the mushroom Lentinus tigrinus. The isolation procedure involved ion exchange chromatography on DEAE-cellulose and CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The laccase was adsorbed on both types of ion ex...

Unbleached kraft pulp fibers were modified with laccase and ferulic acid (FRC) to improve their physical strength properties in paper products. The optimal conditions of laccase-FRC modification were examined in terms of the physical properties of pulps. The effects of laccase-FRC modification on the carboxyl group content and surface lignin content of pulps were investigated. The surface morph...

In this article, the effect of direct current electric field on the phenol biodegradation by laccase was studied. The results showed that the DC electric field could enhance the biodegradation activity of laccase. Specifically, the rate of phenol biodegradation when catalyzed by laccase was significantly higher in the presence of the DC electric field than in its absence. In the presence of the...

Laccase is widely used in several industrial applications and co-culture is a common method for enhancing laccase production in submerged fermentation. In this study, the co-culture of four yeasts with Pleurotus eryngii var. ferulae was found to enhance laccase production. An analysis of sterilization temperatures and extraction conditions revealed that the stimulatory compound in yeasts was te...

BACKGROUND Decolorization of hazardous synthetic dyes using laccases in both free and immobilized form has gained attention during the last decades. The present study was designed to prepare immobilized laccase (purified from Paraconiothyrium variabile) on porous silica beads followed by evaluation of both free and immobilized laccases for decolorization of two synthetic dyes of Acid Blue 25 an...

Enzymes-mediated biocatalysis is believed to be a promising strategy for the degradation of polycyclic aromatic hydrocarbon (e.g., phenanthrene) in aqueous phase, due its high efficiency and environmental harmfulness. However, reusability enzymes-mediated requirement redox mediators limited application. In this paper, enhanced phenanthrene removal was achieved by using novel biocatalyst which c...

Laccase activity and stability were improved and modified through the interaction between laccase amino acid residues (e.g., amino groups (NH2) and carboxyl groups (-COOH)) and the chemical reagents thiourea dioxide (TDO) and L-phenylalanine methyl ester hydrochloride (L-PME). Compared with the control sample, the activity of the laccase modified with these chemical reagents was increased by 20...