L-2-methyltryptophan was prepared from L-serine and 2-methylindole catalyzed by recombinant Escherichia coli with tryptophan synthase activity. In the present research, we used tryptophan synthase (TSase) from recombinant Escherichia coli with pET28a-trpBA plasmid expressing tryptophan synthase from Escherichia coli k-12 MG1655 to produce 2-methyltryptophan, and optimized the conditions for enz...

BACKGROUND L-tryptophan is an important ingredient in medicines, especially in neuromedicines such as antidepressants. Many commercial processes employ various microorganisms with high tryptophan synthase activity to produce L-tryptophan from indole and L-serine, but these processes are very costly due to the costs of precursors, especially L-serine. OBJECTIVES For this reason, we studied the...

L-DOPA, apomorphine and amphetamine increase emotional reactivity and aggressiveness, and/or evoke spontaneous attack behaviour in male rats. At the same time these compounds accelerate the turnover of endogenous serotonin (5-HT), increasing the brain concentration of 5-HIAA. L-DOPA, amphetamine and apomorphine antagonize the depressive effects of 6-tryptophan on behaviour. After the injection ...

L-Tryptophan and some of its analogues were previously observed to bind predominately at one site on human serum albumin (1). The unique nature of this site which favors the binding of L-tryptophan with an association constant 100 times greater than n-tryptophan made it seem worthwhile to investigate the entropy, enthalpy, and free energy changes accompanying the association at this site. In ad...

For biosynthesis of bacillamide C by Bacillus atrophaeus C89 associated with South China sea sponge Dysidea avara, it is hypothesized that decarboxylation from L-tryptophan to tryptamine could be performed before amidation by the downstream aromatic L-amino acid decarboxylase (AADC) to the non-ribosomal peptide synthetases (NRPS) gene cluster for biosynthesizing bacillamide C. The structural an...

For chronic insomniacs, getting a good night’s sleep may appear to be impossible. Fortunately nature provides a natural sleep aid that is both safe and effective. L-tryptophan is an amino acid found in many foods, including turkey. Turkey is well known to cause drowsiness, and the culprit is large amounts of tryptophan. For many years L-tryptophan was the nutritional supplement of choice for ch...