Sporadic isolates of carbapenem-resistant KPC-2-producing Klebsiella pneumoniae were isolated in Tel Aviv Medical Center during 2005 and 2006, parallel to the emergence of the KPC-3-producing K. pneumoniae sequence type 258 (ST 258). We aimed to study the molecular epidemiology of these isolates and to characterize their bla(KPC)-carrying plasmids and their origin. Ten isolates (8 KPC-2 and 2 K...

Klebsiella pneumoniae carbapenemase (KPC)-producing organisms are therapeutically and diagnostically challenging. It is possible that bla(KPC) gene expression plays a role in the variability observed in clinical susceptibility testing. bla(KPC) transformants together with 10 clinical isolates representing four genera were evaluated for bla(KPC) copy number and gene expression and correlated wit...

In the United States, the production of the Klebsiella pneumoniae carbapenemase (KPC) is an important mechanism of carbapenem resistance in Gram-negative pathogens. Infections with KPC-producing organisms are associated with increased morbidity and mortality; therefore, the rapid detection of KPC-producing pathogens is critical in patient care and infection control. We developed a real-time PCR...

Background Carbapenemase-producing Enterobacteriaceae (CPE), including KPC-producing Klebsiella pneumoniae (KPC-Kpn), are an increasing threat to patient safety. Objectives To use WGS to investigate the extent and complexity of carbapenemase gene dissemination in a controlled KPC outbreak. Materials and methods Enterobacteriaceae with reduced ertapenem susceptibility recovered from rectal s...

the objective of this study was to determine the phenotypic characteristics of kpc-producing pseudomonas aeruginosa and acinetobacter baumannii isolates. a case report study was performed at a tertiary burn care centre in tehran,  iran.  nine  isolates  of pseudomonas  aeruginosa  and  acinetobacter baumannii from a hospitalized case were isolated. the identity of isolates was confirmed and the...

Carbapenem resistance due to KPC has rarely been observed outside the United States. We noticed a sharp increase in carbapenem-resistant Klebsiella pneumoniae strains possessing KPC in Tel Aviv Medical Center from 2004 to 2006. Sixty percent of the isolates belonged to a single clone susceptible only to gentamicin and colistin and carried the bla(KPC-3) gene, while almost all other clones carri...

An understanding of the impact of antibiotics on the intestinal reservoir of KPC carbapenemase-producing Klebsiella pneumoniae (KPC-Kp) is important to prevent its emergence. We used a mouse model to examine the effect of antibiotic treatment on the establishment and elimination of intestinal colonization with KPC-Kp. Mice (10 per group) received subcutaneous antibiotics daily for 8 days. On da...

conclusions the incidence of mbls producing p. aeruginosa in patients with cf was not low. the findings indicate that the identification of drug-resistance patterns in p. aeruginosa and the detection of mbl-producing isolates are important in the prevention and control of infections. background respiratory infections caused by pseudomonas aeruginosa play an important role in the pathogenesis of...

introduction klebsiella pneumoniae is one of the most important gram-negative bacteria causing hospital-acquired infections. case presentation in this report is presented a patient with an abdominal infection caused by carbapenemase producing-klebsiella pneumonia (k. pneumonia). in spite of administrating the combination therapy, successive resistance to last-resort antimicrobial agents (colist...