نتایج جستجو برای: knock out serum replacement
تعداد نتایج: 1209402 فیلتر نتایج به سال:
With the completion of genome sequences of major model organisms, increasingly sophisticated genetic tools are necessary for investigating the complex and coordinated functions of genes. Here we describe a genetic manipulation system termed "genomic engineering" in Drosophila. Genomic engineering is a 2-step process that combines the ends-out (replacement) gene targeting with phage integrase ph...
We demonstrate in this article the rapid determination of heterozygous and homozygous transgenic knock-out mice using the polymerase chain reaction (PCR) with three primers. A similar technique was described by Horton et al. (3), which demonstrated threeprimer PCR in the genotype determination of knock-out RAG-2 immunodeficient mice. However novel this approach may be, one must conclude that th...
OBJECTIVE To understand the mechanisms of skeletal muscle destruction and resistance to enzyme replacement therapy in Pompe disease, a deficiency of lysosomal acid alpha-glucosidase (GAA), in which glycogen accumulates in lysosomes primarily in cardiac and skeletal muscles. METHODS We have analyzed compartments of the lysosomal degradative pathway in GAA-deficient myoblasts and single type I ...
Previous studies with peritoneal macrophages obtained from macrophage scavenger receptor class A (MSR-A) knock-out mice showed that the endocytic uptake of advanced glycation end products (AGE) by macrophages was mediated mainly by MSR-A. However, it is controversial whether the endocytic uptake of intravenously injected AGE proteins by liver sinusoidal endothelial cells (LECs) is similarly exp...
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